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Alexa Fluor® 647 anti-ApoE Antibody

Pricing & Availability
Clone
D6E10 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. D6E10_647_ApoE_IHC-P_091725
IHC staining with Alexa Fluor® 647 anti-ApoE (clone D6E10) on formalin-fixed paraffin-embedded human Alzheimer’s Brain diseased cortex. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Alexa Fluor® 647 anti-ApoE (clone D6E10). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • A. D6E10_647_ApoE_IHC-P_091725
    IHC staining with Alexa Fluor® 647 anti-ApoE (clone D6E10) on formalin-fixed paraffin-embedded human Alzheimer’s Brain diseased cortex. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Alexa Fluor® 647 anti-ApoE (clone D6E10). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • B. D6E10_647_ApoE_ICC_091725
    HeLa cells (negative cell line, panel A) and HepG2 cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Alexa Fluor® 647 anti-ApoE (clone D6E10). Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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803309 25 µg 156€
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803310 100 µg 376€
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-100% ice-cold methanol only
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol 
Fixation/permeabilization using Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 is not recommended.

Application References
  1. Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72.
  2. Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108.
  3. Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8.
  4. Golabek AA, et al. 2000. Biophys J. 79(2):1008-15.
  5. Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8.
  6. Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6.
  7. Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79.
  8. Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
RRID
AB_3720618 (BioLegend Cat. No. 803309)
AB_3720618 (BioLegend Cat. No. 803310)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.17.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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