Ultra-LEAF™ Purified anti-human CD45 (Flexi-Pure™) Antibody, CD45, HI30

Pricing & Availability

Clone: HI30 (See other available formats)
Regulatory Status: RUO
Workshop: IV N816
Other Names: LCA, T200
Isotype: Mouse IgG1, κ
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Product Citations: publications

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285763

1 mg

$430

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285764

5 mg

$1663

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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 4 mg/mL and 10 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC
Recommended Usage: Our Ultra-Low Endotoxin, Azide-Free (Ultra-LEAF™) formulation is designed for in vitro and in vivo cellular and animal model applications. The antibodies are 0.2 µm filtered and do not contain any preservatives (azide free). It is recommended that the reagent be titrated for optimal performance.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections⁹, inhibition of CD45 functions⁴, immunofluorescence¹¹, Western blotting³, and spatial biology (IBEX)^16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Additional Product Notes: Flexi-Pure™ products are rapidly-made custom purified antibodies.

Antigen Details

Structure: Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution: Hematopoietic cells, not expressed in circulating erythrocytes or platelets
Function: TCR and BCR mediated activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: Thomas M. 1989. Annu. Rev. Immunol. 7:339.

Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID: NA
UniProt: View information about CD45 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
What are Flexi-Pure™ antibodies?: Flexi-Pure are rapidly made, made-to-order purified antibodies that are more concentrated than our traditional Ultra-LEAF antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Pure are the same as that of Ultra-LEAF.
How quickly will I receive my Flexi-Pure™ antibody order?: We aim to ship the Flexi-Pure antibodies within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
How do I determine the optimal concentration for using my Flexi-Pure™ antibodies? How should I titrate my antibody?: Flexi-Pure antibodies are provided at concentrations between 4 - 10 mg/mL. We recommend that you titrate your antibody to determine the optimal concentration to use for your application.
Are the specifications of Ultra-LEAF™ and Flexi-Pure™ antibodies the same?: Yes, they are the same. For example, neither Ultra-LEAF, nor Flexi-Pure antibodies are tested for pathogens. In addition, they are not tested for functional applications unless indicated otherwise in their respective Technical Datasheet. However, there are differences in concentration. Typically, Flexi-Pure™ antibodies are more concentrated than the Ultra-LEAF™ antibodies.
I can’t find the Flexi-Pure™ antibody I need. When will it be available?: Please contact your local Field Applications Scientist.
I need help to validate the performance of my Flexi-Pure™ antibody. Who should I contact?: Please get in touch with Technical Service for assistance.
Can I order more sizes other than 1 mg or 5 mg of Flexi-Pure™?: Yes, for bulk single-lot orders, contact our Custom Solutions team.
What is the expiration date of my Flexi-Pure™ antibody?: Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
APC/Cyanine7 anti-human CD45	HI30	FC
PE/Cyanine7 anti-human CD45	HI30	FC
Alexa Fluor® 594 anti-human CD45	HI30	IHC-P,SB
APC/Fire™ 750 anti-human CD45	HI30	FC
Pacific Blue™ anti-human CD45	HI30	FC
APC anti-human CD45	HI30	FC
PE/Dazzle™ 594 anti-human CD45	HI30	FC
PE/Cyanine7 anti-human CD45	HI30	FC
TotalSeq™-A0391 anti-human CD45	HI30	PG
TotalSeq™-B0391 anti-human CD45	HI30	PG
TotalSeq™-C0391 anti-human CD45	HI30	PG
PerCP/Cyanine5.5 anti-human CD45	HI30	FC
APC/Fire™ 750 anti-human CD45	HI30	FC
PE/Fire™ 640 anti-human CD45	HI30	FC
APC/Fire™ 810 anti-human CD45	HI30	FC
Spark YG™ 570 anti-human CD45	HI30	IHC-P
PE/Fire™ 700 anti-human CD45	HI30	FC
FITC anti-human CD45	HI30	FC
PerCP anti-human CD45	HI30	FC
FITC anti-human CD45	HI30	FC
Purified anti-human CD45	HI30	FC,CyTOF®,IHC-P,WB,IHC-F,ICC
PE/Cyanine5 anti-human CD45	HI30	FC
PE anti-human CD45	HI30	FC
Biotin anti-human CD45	HI30	FC
APC anti-human CD45	HI30	FC
Alexa Fluor® 488 anti-human CD45	HI30	FC
Alexa Fluor® 647 anti-human CD45	HI30	FC
Pacific Blue™ anti-human CD45	HI30	FC
Alexa Fluor® 700 anti-human CD45	HI30	FC
PerCP anti-human CD45	HI30	FC
PerCP/Cyanine5.5 anti-human CD45	HI30	FC,SB
Brilliant Violet 421™ anti-human CD45	HI30	FC
Brilliant Violet 570™ anti-human CD45	HI30	FC
Brilliant Violet 510™ anti-human CD45	HI30	FC
Brilliant Violet 605™ anti-human CD45	HI30	FC
Brilliant Violet 650™ anti-human CD45	HI30	FC
Purified anti-human CD45 (Maxpar® Ready)	HI30	FC,CyTOF®
Brilliant Violet 785™ anti-human CD45	HI30	FC
Brilliant Violet 711™ anti-human CD45	HI30	FC
PE/Dazzle™ 594 anti-human CD45	HI30	FC
Alexa Fluor® 660 anti-human CD45 Antibody	HI30	FC
Spark Violet™ 538 anti-human CD45	HI30	FC
Spark YG™ 593 anti-human CD45	HI30	FC
GMP APC/Fire™ 750 anti-human CD45	HI30	FC
Spark Violet™ 538 anti-human CD45	HI30	FC
GMP APC anti-human CD45	HI30	FC
Spark UV™ 387 anti-human CD45	HI30	FC
GMP Pacific Blue™ anti-human CD45	HI30	FC
GMP PerCP anti-human CD45	HI30	FC
GMP FITC anti-human CD45	HI30	FC
PE anti-human CD45	HI30	FC
GMP PE/Dazzle™ 594 anti-human CD45	HI30	FC
GMP PerCP/Cyanine5.5 anti-human CD45	HI30	FC
Spark Blue™ 515 anti-human CD45	HI30	FC
TotalSeq™-D0391 anti-human CD45	HI30	PG
GMP PE/Cyanine7 anti-human CD45	HI30	FC
Spark Violet™ 500 anti-human CD45	HI30	FC
GMP PE anti-human CD45	HI30	FC
PE/Fire™ 810 anti-human CD45	HI30	FC
Spark PLUS UV395™ anti-human CD45	HI30	FC
Spark NIR™ 685 anti-human CD45	HI30	FC
Spark Violet™ 423 anti-human CD45	HI30	FC
GMP Spark Violet™ 538 anti-human CD45	HI30	FC
PE/Cyanine5 anti-human CD45	HI30	FC
Spark YG™ 581 anti-human CD45 (Flexi-Fluor™)	HI30	FC
PE/Fire™ 744 anti-human CD45	HI30	FC
Spark PLUS B550™ anti-human CD45 Antibody	HI30	FC
Spark PLUS V475™ anti-human CD45 Antibody	HI30	FC
Ultra-LEAF™ Purified anti-human CD45 (Flexi-Pure™)	HI30	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/17/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.