Loading...

Purified anti-Bcl-XL Antibody

Pricing & Availability
Clone
W23168D (See other available formats)
Regulatory Status
RUO
Other Names
BCLX, BCL-X, Bcl-2-like protein 1, BCL2L1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A. W23168D_PURE_Bxl-XL_WB_063025
Whole cell extracts (15 µg total protein per lane) from HAP1 WT cells (Horizon Cat. No. C631) or HAP1 BCL2L1 (Bcl-X) Knockout cells (Horizon Cat. No. HD HAP1-65076) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Bcl-XL (clone W23168D). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • A. W23168D_PURE_Bxl-XL_WB_063025
    Whole cell extracts (15 µg total protein per lane) from HAP1 WT cells (Horizon Cat. No. C631) or HAP1 BCL2L1 (Bcl-X) Knockout cells (Horizon Cat. No. HD HAP1-65076) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Bcl-XL (clone W23168D). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • B. W23168D_PURE_Bxl-XL_ICC_063025
    IHC staining of BCL-XL was performed on formalin-fixed paraffin-embedded human prostate cancer tissue following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated without (panel A) or with anti-BCL-XL (clone W23168D) (panel B and C) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
  • C. W23168D_PURE_Bxl-XL_ICFC_063025
    HAP1 WT cells (positive cell line, filled histogram) (Horizon Cat. No. C631) and HAP1 BCL2L1 (Bcl-X) Knockout cells (negative cell line, open histogram) (Horizon Cat. No. HAP1-65076) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Purified anti-BCL-XL (clone W23168D) or Purified Rat IgG2a Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) (Cat No. 400501) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
Cat # Size Price Quantity Check Availability Save
648807 25 µg $170
Check Availability


Need larger quantities of this item?
Request Bulk Quote
648808 100 µg $445
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Bcl-X, a member of the Bcl-2 family of proteins, is encoded by the BCL2L1 gene and plays a crucial role in regulating apoptosis. Bcl-X exists in two main isoforms: Bcl-XL, which is anti-apoptotic, and Bcl-XS, which is pro-apoptotic. The balance between these isoforms is regulated through alternative splicing, influenced by various splice factors, transcription factors, and signaling pathways. Dysregulation of Bcl-X splicing has been implicated in several diseases, particularly cancer and diabetes. In cancer, overexpression of Bcl-XL can lead to resistance to chemotherapy, while in diabetes, increased Bcl-XS expression can result in apoptosis of pancreatic β-cells.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company:
HAP1 WT Cell Line (Horizon Cat No. Cat. No. C631) and HAP1 BCL2L1 Knockout cell line (Horizon Cat. No. HD HAP1-65076)

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Bcl-X protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Due to homology, this antibody may detect the isoform Bcl-XS (18.8 kDa) which is a result of alternative splicing. However, in Western Blot, this isoform was not detected. The canonical sequence, isoform Bcl-XL, was detected by this antibody in Western Blot.

Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization either using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803).

We DO NOT recommend using True-Phos™ Perm Buffer (Cat. No. 425401).

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

 

RRID
AB_3717173 (BioLegend Cat. No. 648807)
AB_3717173 (BioLegend Cat. No. 648808)

Antigen Details

Structure
Bcl-XL is a 233 amino acid length protein with a molecular weight of approximately 26 kDa.
Distribution

Mitochondrial membrane and cytoplasm

Interaction
Bcl-XL and Bcl-XS interact with: Bad, Bak, Bim, Bid, Bax and p53
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Death, Cell Proliferation and Viability
Molecular Family
Adaptor Proteins, Mitochondrial Markers, Scaffold Proteins
Antigen References
  1. Dou Z, et al. 2021. J Exp Clin Cancer Res. 40, 194.
  2. Bharatham N, et al. 2011. PLOS One. doi.org/10.1371/journal.pone.0026014.
  3. Stevens M, et al. 2019. Front Genet.10:doi.org/10.3389/fgene.2019.00804.
  4. Findley HW, et al. 1997. Blood. 89:2986.
Gene ID
598 View all products for this Gene ID
UniProt
View information about Bcl-XL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/30/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account