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PE anti-mouse CXCL2 (MIP-2) Antibody

Pricing & Availability
Clone
A21113C (See other available formats)
Regulatory Status
RUO
Other Names
Gro2 oncogene, Groβ, Mip-2, Mip2, Scyb2, chemokine (C-X-C motif) ligand 2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. A21113C_PE_CXCL2_ICFC_1_062725-2
LPS-stimulated (6 hours, with monensin) C57BL/6 mouse bone marrow cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No.426803) and intracellularly stained with anti-mouse/human CD11b (clone M1/70) APC and anti-mouse CXCL2 (MIP-2) (clone A21113C) PE (left) or rat IgG2b, κ PE isotype control (right).
  • a. A21113C_PE_CXCL2_ICFC_1_062725-2
    LPS-stimulated (6 hours, with monensin) C57BL/6 mouse bone marrow cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No.426803) and intracellularly stained with anti-mouse/human CD11b (clone M1/70) APC and anti-mouse CXCL2 (MIP-2) (clone A21113C) PE (left) or rat IgG2b, κ PE isotype control (right).
  • b. A21113C_PE_CXCL2_ICFC_2_062725-2
    Unstimulated C57BL/6 mouse bone marrow cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No. 426803) and intracellularly stained with anti-mouse/human CD11b (clone M1/70) APC and anti-mouse CXCL2 (MIP-2) (clone A21113C) PE (left) or rat IgG2b, κ PE isotype control (right).
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168503 25 µg $214
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168504 100 µg $435
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Description

CXCL2 is an ELR CXC chemokine that is structurally and functionally related to GRO1 (CXCL1), GRO3 (CXCL3), and interleukin-8 (CXCL8). CXCL2 binds to the CXCR2 receptor, which is shared with other ELR CXC chemokines, including CXCL1, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8. CXCL2 is highly expressed in esophageal squamous cell carcinoma (ESCC), and has been detected in the serum of patients with this disease. Elevated levels of CXCL1, CXCL2 and CXCL3 have also been observed in patients with malignant melanoma.

In addition, CXCL2 and CXCL1 are upregulated in the central nervous system in multiple sclerosis (MS) and in the animal model of experimental autoimmune encephalomyelitis (EAE). In EAE, the transfer of encephalitogenic CD4+ Th17 cells induces CXCL1 and CXCL2 transcription in the spinal cords of naïve, syngeneic recipients. IL-17 is a potent inducer of ELR+ CXC chemokines in both MS and EAE, with CXCL1 and CXCL2 shown to be upregulated in the preclinical and clinical stages of EAE. Additionally, CXCL1 and CXCL2 are secreted by epithelial cells in the bone marrow, while CXCL2 is secreted by endothelial cells during G-CSF-induced neutrophil mobilization.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse CXCL2 (MIP-2)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Recombinant protein partially blocks the staining of CXCL2 on BM cells. 

Antigen Details

Structure
7.8 kD protein (73 amino acids), member of the CXC chemokine family
Distribution

Macrophages, monocytes, fibroblasts, intestinal enterocytes, bone marrow endothelial cells, osteoblasts, and epithelial cells

Function
CXCL2 chemoattracts and activates neutrophils, and it plays a key role in neutrophil mobilization from the bone marrow. CXCL2 is induced by TNFα, IL-1α, IL-17, and LPS.
Interaction
Neutrophils, basophils, lymphocytes, monocytes, keratinocytes, hematopoietic cells, endothelial cells, bone marrow endothelial cells, osteoblasts, epithelial cells, fibroblasts, melanoma cells, melanocytes, and neurons
Ligand/Receptor
CXCR2
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Haskill S, et al. 1990. P. Natl. Acad. Sci. USA. 87:7732.
  2. De Plaen I, et al. 2006. Immunology. 118:153.
  3. Carlson T, et al. 2008. J. Exp. Med. 205:811.
  4. Eash KJ, et al. 2010. J. Clin. Invest. 120:2423.
  5. Keeley EC, et al. 2011. Exp. Cell. Res. 317:685.
  6. Onishi RM and Gaffen SL. 2011. Immunology. 129:311.
Gene ID
20310 View all products for this Gene ID
UniProt
View information about CXCL2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/27/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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