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- Regulatory Status
- RUO
- Other Names
- Multiplex IHC Kit, Multiplex IHC TSA Amplification Kit, StreptaClick®–HRP
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- Product Citations
- publications
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Multiplex immunostaining staining on frozen spleen tissue from C57BL/6 mice. First, the tissue sections were fixed with 4% paraformaldehyde for 10 minutes and blocked. Then, the tissue was stained with the Multiplex Immunohistochemistry Kit (3-color TSA) along with 3 biotinylated antibodies (panel B) or buffer only (negative control, panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) and images were merged (panels A and B). The image was captured using a 10X objective. The tyramide dye and antibody combinations, exposure times and cycle number are noted in panel C. -
| Cat # | Size | Price | Quantity | Save | ||
|---|---|---|---|---|---|---|
| 426503 | 90 tests | $995 | ||||
| 426504 | 250 tests | $1495 | ||||
BioLegend’s Multiplex Immunohistochemistry Kit (3-color TSA) provides a powerful method for multiplex Tyramide Signal Amplification (TSA) on frozen or formalin-fixed paraffin embedded (FFPE) tissues. This kit uses click reaction to attach StreptaClick®–HRP, a monovalent horseradish peroxidase (HRP), to your biotinylated antibody of choice. The HRP-labeled biotinylated antibodies can then be used for multiple cycles of immunostainings where each fluorochrome is sequentially developed by TSA. This kit utilizes a novel HRP Block Buffer that efficiently quenches HRP activity between staining cycles without the need for heat treatment. This results in a faster and streamlined protocol for multiplex immunostaining experiments with reliable results.
StreptaClick®–HRP is compatible with biotinylated antibodies from any host and on tissues from mice, humans, rats, and other species. The kit includes 3 Tyramide dyes: Tyramide 488, Tyramide 555, and Tyramide 647.
Click here to view BioLegend’s biotin-labeled antibodies which are verified for use in immunohistochemistry.
Product Details
- Verified Reactivity
- Species independent
- Preparation
- Please refer to the detailed protocol in the Related Protocols section.
- Storage & Handling
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Kit Contents (90/250 tests)
3% H2O2 (150/300 µL) - Store at 4°C
Biotin Block Buffer (300/600 µL) - Store at 4°C
HRP Block Buffer (9/25 mL) - Store at 4°C
Block Activator (1450/1450 µL) - Store at 4°C
StreptaClick®–HRP (170/500 µL) - Store at 4°C
TSA Amplification Buffer Plus (25 mL) - Store at 4°C
30% H2O2 (100 µL) - Store at 4°C
Tyramide 488 (80 µg) - Store at -20°C
Tyramide 555 (80 µg) - Store at -20°C
Tyramide 647 (80 µg) - Store at -20°C - Application
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IHC-F - Quality tested
IHC-P - Verfiied - Recommended Usage
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Please see the detailed protocol below in the Related Protocols section.
- Application Notes
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This kit can be used for multiplex immunohistochemistry using Tyramide Signal Amplification (TSA) on frozen or formalin-fixed paraffin embedded (FFPE) tissue sections. The protocol does not use heat treatment between cycles, which also allows TSA multiplex immunostaining on frozen tissue sections.
This kit uses click reaction to attach monovalent horseradish peroxidase (HRP) to your biotinylated antibody of choice. The HRP-labeled biotinylated antibodies can then be used for multiple cycles of immunostainings where each fluorochrome is sequentially developed by TSA. The kit contains an HRP Block Buffer optimized for preserving the morphology of frozen tissue sections. The HRP block buffer rapidly quenches the HRP enzyme after each TSA cycle and allows for sequential multiplex IHC.
Please see the detailed protocol below:
https://www.biolegend.com/en-us/protocols/multiplex-immunohistochemistry-kit-3-color-tsa-protocol
Click here to view BioLegend’s biotin-labeled antibodies which are verified for use in immunohistochemistry. - Additional Product Notes
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Name Absoption Max Emission Max Tyramide 488 494 nm 517 nm Tyramide 555 555 nm 572 nm Tyramide 647 649 nm 671 nm
Tyramide Dyes:
Fluorescence tyramide dyes enable tyramide signal amplification (TSA), delivering exceptional signal strength for immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH) applications.
How TSA Works:
TSA leverages horseradish peroxidase (HRP) chemistry to achieve dramatic signal enhancement. In the presence of hydrogen peroxide, HRP catalyzes the conversion of tyramide-containing dyes into highly reactive free radicals. These activated molecules covalently bind to tyrosine residues at or near the HRP location, creating permanent, amplified signals directly at your target site.
Antigen Details
- Gene ID
- NA
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