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Ultra-LEAF™ Purified anti-human CCL1 (I-309) Antibody

Pricing & Availability
Clone
A16001D (See other available formats)
Regulatory Status
RUO
Other Names
T lymphocyte-secreted protein I-309
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A16001D_ULEAF_CCL1_Block_052325
Recombinant human CCL1 (I-309) (Cat. No. 582702) (black circles) induces the chemotaxis of mouse Baf3-hCCR8 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL1 (I-309) (clone A16001D) (purple squares) neutralizes the chemotaxis of recombinant human CCL1 (I-309) at 0.6 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner, whereas the Ultra-LEAF™ Purified Mouse IgG1, k Isotype Control (Cat. No. 401408) (gray triangles) does not have the effect. ND50 range: 0.1 – 1 μg/mL.
  • A16001D_ULEAF_CCL1_Block_052325
    Recombinant human CCL1 (I-309) (Cat. No. 582702) (black circles) induces the chemotaxis of mouse Baf3-hCCR8 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CCL1 (I-309) (clone A16001D) (purple squares) neutralizes the chemotaxis of recombinant human CCL1 (I-309) at 0.6 ng/mL on mouse Baf3-mCXCR2 cells in a dose-dependent manner, whereas the Ultra-LEAF™ Purified Mouse IgG1, k Isotype Control (Cat. No. 401408) (gray triangles) does not have the effect. ND50 range: 0.1 – 1 μg/mL.
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665853 100 µg 256€
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665854 1 mg 592€
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Description

CCL1 was initially identified as a secreted protein derived from activated T cells. CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation. As a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and, compared to normal patients, higher concentration of CCL1 is observed in bronchoalveolar lavage of asthmatic patients. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication demonstrated that in in-vitro conditions, CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β. Furthermore, this transcriptional upregulation is reversed could be reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Also, antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CCL1 (I-309)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the chemotaxis of mouse Baf3- hCCR8 cells induced by recombinant human CCL1 (I-309) (Cat. No. 582702) at 0.6 ng/mL. ND50 range: 0. 1- 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3717089 (BioLegend Cat. No. 665853)
AB_3717089 (BioLegend Cat. No. 665854)

Antigen Details

Structure
Chemokine
Distribution

T cells, Th1, Th2, diabetogenic T cells, peritoneal macrophages, peritoneal mesothelial cells, Langerhans cells, monocytes, mast cells, NK cells, endothelial cells, and epithelial cells

Function
CCL1 chemoattracts CD4 Th2 cells and plays a key role in the progression of Th2 type hypersensitivity.
Interaction
T cells, Th2 cells, macrophages, microglia, FOXP3+ CD4 regulatory T cells, skin-homing CLA+T cells, monocytes, NK cells, dendritic cells, endothelial cells, and smooth muscle cells
Ligand/Receptor
CCR8 (CDw198)
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Miller MD, et al. 1989. J Immunol. 143:2907-16.
  2. Haque NS, et al. 2004. Blood. 103:1296-304
  3. Gombert M, et al. 2005. J Immunol. 174:5082-91.
  4. Montes-Vizuet R, et al. 2006. Eur Respir J. 28:59-67.
  5. Soler D, et al. 2006. J Immunol. 177:6940-51.
  6. Gonzalo JA, et al. 2007. J Immunol. 179:1740-50.
  7. Mutalithas K, et al. 2010. Clin Exp Allergy. 40:1175-85.
Gene ID
6346 View all products for this Gene ID
UniProt
View information about CCL1 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 05/23/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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