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Purified anti-SDHA Antibody

Pricing & Availability
Clone
W23126E (See other available formats)
Regulatory Status
RUO
Other Names
succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF
Isotype
Rat IgG2a, κ
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Product Citations
publications
A. W23126E_PURE_SDHA_WB_060925
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-SDHA (clone W23126E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W23126E_PURE_SDHA_WB_060925
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with Purified anti-SDHA (clone W23126E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W23126E_PURE_SDHA_IHC-P_1_060925
    IHC staining with Purified anti-SDHA (clone W23126E) was performed on FFPE human heart tissue following antigen retrieval using 1X Citrate buffer (pH 6.0) (Cat. No. 420902). The tissue sections were incubated with 5 µg/mL of Purified anti-SDHA (clone W23126E) (panels B and C) followed by Alexa Fluor® 594 Goat anti-rat (Cat. No. 405422) (red) or secondary antibody only (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) (merged in panels A and C). The image was captured with a 40X objective. Scale Bar: 50 µm
  • c. W23126E_PURE_SDHA_IHC-P_2_060925.
    IHC staining with Purified anti-SDHA (clone W23126E) was performed on FFPE human kidney tissue following antigen retrieval using 1X Citrate buffer (pH 6.0) (Cat. No. 420902). The tissue sections were incubated with 5 µg/mL of Purified anti-SDHA (clone W23126E) (panels B and C) followed by Alexa Fluor® 594 Goat anti-rat (Cat. No. 405422) (red) or secondary antibody only (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) (merged in panels A and C). The image was captured wit a 40X objective. Scale Bar: 50 µm
  • d. W23126E_PURE_SDHA_ICC_060925
    HeLa cells treated with mock shRNA (panel B) or knockdown with transiently transfected SDHA shRNA (panel C) were fixed with fixation buffer (Cat. No. 420801) for 10 minutes. Then, permeabilized with ice-cold methanol and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with isotype control (Cat. No. 400602) (panel A) or stained with Purified anti-SDHA (clone W23126E), followed by incubation with Alexa Fluor 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Then, cells were co-stained with Alexa Fluor® 488 anti-GFP Antibody (Cat. No. 338008) to detect expression of shRNA reporter for 1 hour at room temperature (panel C). Nuclei were counterstained with DAPI (Cat. No. 422801). The image was captured with a 40X objective. Scale bar: 50 µm
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648451 25 µg 136€
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648452 100 µg 364€
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Description

Succinate dehydrogenase (SDH), commonly known as respiratory complex II, is an important enzyme of mitochondria in the citric acid cycle and electron transport chain, and consists of four subunits SDHA, SDHB, SDHC, and SDHD. SDHA, like other SDHx genes, can function as a tumor suppressor gene. Recently, studies have indicated that mutations in SDHA contribute to various disorders such as hereditary pheochromocytoma-paraganglioma (PPGL) syndrome, mitochondrial complex II deficiency, gastrointestinal stromal tumors, Leigh syndrome, dilated cardiomyopathy, and neuroendocrine tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human SDHA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

This antibody has been tested in knockout/knockdown systems for WB and ICC.

This antibody (clone W23126E) does not cross-react with mouse SDHA on Western Blot (WB).

For immunocytochemistry (ICC), we recommend fixation with 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation.

For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) is recommended.

RRID
AB_3717114 (BioLegend Cat. No. 648451)
AB_3717114 (BioLegend Cat. No. 648452)

Antigen Details

Structure
SDHA is a 664 amino acid protein with a predicted molecular weight of 73 kD.
Distribution

Membrane and mitochondrion inner membrane

Function
An important enzyme of mitochondria in the citric acid cycle and electron transport chain, tumor suppressor gene, prognostic marker
Interaction
Interacts with SDHAF2/SDH5, TRAP1, LACC1.
Biology Area
Cardiovascular Biology, Cell Biology, Cell Death, Cell Proliferation and Viability, Neurodegeneration, Neuroscience
Molecular Family
Mitochondrial Markers, Tumor Suppressors
Antigen References
  1. Oyedotun KS, et al. 2004. J Biol Chem. 279: 9424.
  2. Rutter J, et al.2011. Mitochondrion.10:393.
  3. Burnichon N. 2010. Hum Mol Genet.19: 3011.
  4. Benchoua A, et al. 2006. Mol Biol Cell. 17:1652.
Gene ID
6389 View all products for this Gene ID
UniProt
View information about SDHA on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

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Description Clone Applications
Purified anti-SDHA W23126E WB,ICC,IHC-P
Go To Top Version: 0    Revision Date: 01/01/0001

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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