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Purified anti-CDC2/CDK1 Phospho (Thr161) Antibody

Pricing & Availability
Clone
A23004F (See other available formats)
Regulatory Status
RUO
Other Names
CDC2: Cell Division Cycle 2 homolog12, CDC28A, P34CDC2, Cell Division Protein Kinase 1:
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A23004F_PURE_CDC2_WB_062425
HeLa cells were treated (+) or untreated (-) with Nocodazole to arrest the cell cycle at G2/M. Whole cell extracts (15 µg total protein per lane) from the cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with either Purified pan anti-CDC2/CDK1 (left panel) or Purified anti-CDC2/CDK1 Phospho (Thr161) (clone A23004F) (right panel). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • A. A23004F_PURE_CDC2_WB_062425
    HeLa cells were treated (+) or untreated (-) with Nocodazole to arrest the cell cycle at G2/M. Whole cell extracts (15 µg total protein per lane) from the cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with either Purified pan anti-CDC2/CDK1 (left panel) or Purified anti-CDC2/CDK1 Phospho (Thr161) (clone A23004F) (right panel). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • B. A23004F_PURE_CDC2_IHC-P_062425
    IHC staining with Purified anti-CDC2/CDK1 Phospho (Thr161) (clone A23004F) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated with Alexa Fluor® 647 secondary antibody only (panel A) or with Purified anti-CDC2/CDK1 Phospho (Thr161) (clone A23004F) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (panel B and C). Nuclei were counterstained with DAPI (Cat. No. 422801) (Overlay in panels A and C). Scale bar: 50 µm.
  • C. A23004F_PURE_CDC2_ICFC-1_062425
    Flow cytometric analysis using Purified anti-CDC2/CDK1 Phospho (Thr161) (clone A23004F) HeLa cells treated with Nocodazole (to arrest cell cycle at G2/M) were fixed and permeabilized using True-Phos™ Perm Buffer Set (Cat. No. 425401) and co-stained with DAPI (Cat. No. 422801) to determine the cell cycle phase. The cells were intracellularly stained with Purified anti-CDC2/CDK1 Phospho (Thr161) (clone A23004F, purple filled and solid line histograms) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 400102) (dashed line histogram) followed by Alexa Fluor® 647 Goat anti-mouse (Cat No. 405322) for secondary detection. The flow cytometric analysis was done by comparing cells in G0/G1 (low CDC2/CDK1 phosphorylation, solid line histogram) versus cells in G2/M (high CDC2/CDK1 phosphorylation, purple filled histogram).
  • D. A23004F_PURE_CDC2_ICFC-2_070925-2
    HeLa cells treated with Nocodazole to arrest cell cycle at G2/M, were fixed and permeabilized using the True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-CDC2/CDK1 Phospho (Thr161) (clone A23004F) (right panel) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 400102) (left panel), followed by Alexa Fluor® 647 Goat anti-mouse (Cat No. 405322). Cell cycle analysis was performed by DAPI staining (Cat. No. 422801).
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649151 25 µg 116€
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649152 100 µg 324€
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Description

CDC2, also known as CDK1 or Cyclin-Dependent Kinase 1, is a crucial regulator of G2 phase progression to mitosis in the cell cycle. The phosphorylation of CDC2 at Threonine 161 (Thr161) is essential for its activation. This phosphorylation is carried out by the CDK-activating kinase (CAK) and is necessary for CDC2 to form an active complex with cyclin B1, promoting mitosis. The regulation of CDC2 activity involves a delicate balance of phosphorylation and dephosphorylation events. In maintaining this balance, kinases like WEE1 and MYT1 inhibit CDC2 by phosphorylating other residues. Meanwhile, phosphatases like CDC25 activate CDC2 by removing these inhibitory phosphates. Dysregulation of CDC2, including aberrant phosphorylation at Thr161, is associated with various cancers, such as breast, liver, and colon cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human CDK1 phosphorylated at threonine 161
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has cross reactivity with CDK2 phospho (Thr160) (verified), CDK3 phospho (Thr172) (verified), and CDK7 phospho (Thr170) (expected, not verified) due to the high homology/identity with CDK1 phospho (Thr161) site.

Additional Product Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).

For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).

RRID
AB_3717197 (BioLegend Cat. No. 649151)
AB_3717197 (BioLegend Cat. No. 649152)

Antigen Details

Structure
CDC2 is 297 amino acids length protein with a molecular weight of approximately 34 kDa.
Distribution

Nucleoplasm and cytoplasm

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Morgan D. 1995. Nature. 374:131.
  2. Massacci G, et al. 2023. British Journal of Cancer. 129:1707.
  3. Enserink JM, et al. 2010. Cell Division. 5:1.
  4. Cho Y, et al. 2025. Cell Death Dis. 16:202.
Gene ID
983 View all products for this Gene ID
UniProt
View information about CDC2/CDK1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/24/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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