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Ultra-LEAF™ Purified anti-human Thrombopoietin Antibody

Pricing & Availability
Clone
A22080G (See other available formats)
Regulatory Status
RUO
Other Names
Megakaryocyte Colony Stimulating Factor (MKCSF), Myeloproliferative leukemia virus
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18102K_ULEAF_TWEAK_Neut_082825-2
Recombinant human TPO (Cat. No. 763702) (black circles) induces proliferation of MO7e cell. Ultra- LEAF™ purified anti- human Thrombopoietin (A22080G) (purple squares) neutralizes the MO7e cell proliferation induced by recombinant human TPO at 1 ng/mL in a dose-dependent manner, whereas the Ultra- LEAF™ purified mouse IgG2b, κ Isotype Control (Cat. No. 401216) (gray triangles) does not have this effect. ND50 range: 0.2 - 2 µg/mL.
  • A18102K_ULEAF_TWEAK_Neut_082825-2
    Recombinant human TPO (Cat. No. 763702) (black circles) induces proliferation of MO7e cell. Ultra- LEAF™ purified anti- human Thrombopoietin (A22080G) (purple squares) neutralizes the MO7e cell proliferation induced by recombinant human TPO at 1 ng/mL in a dose-dependent manner, whereas the Ultra- LEAF™ purified mouse IgG2b, κ Isotype Control (Cat. No. 401216) (gray triangles) does not have this effect. ND50 range: 0.2 - 2 µg/mL.
Cat # Size Price Quantity Check Availability Save
648854 100 µg £202
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648855 1 mg £472
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Description

TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. TPO binds to its receptor, the cellular homologue of the myeloproliferative leukemia virus oncogene (c-Mpl) and stimulates the proliferation and maturation of megakaryocytes. MPL does not possess kinase activity, thus the receptor associates with intracytoplasmic tyrosine kinases, janus kinase 2 (JAK2), for signal transduction.  JAK2 is also important for MPL stability and cell-surface expression. TPO can promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multi-lineage progenitor cells. TPO levels in blood and bone marrow are inversely related to platelet count.  The regulation of TPO levels is mediated by its receptor c-Mpl (uptake and destruction). TPO is upregulated by PDGF and FGF-2, and it is downregulated by PF4, thrombospondin, and TGF-β in bone marrow stromal cells. In hepatocytes, HGF enhances TPO mRNA expression. During acute-phase response, IL-6 induces TPO transcription in the liver. Elevated plasma TPO levels exist in several hematological diseases associated with thrombocytopenia, coronary syndromes, and sepsis. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling. Increased TPO concentrations are present in the cerebrospinal fluid of some patients with bacterial or viral meningitis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human TPO
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the proliferation induced by recombinant human TPO (Cat. No. 763702) at 1 ng/mL in the MO7e cells. ND50 range: 0.2 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3720579 (BioLegend Cat. No. 648854)
AB_3720579 (BioLegend Cat. No. 648855)

Antigen Details

Structure
Growth Factor, homodimer
Distribution

TPO is produced by the liver and kidney and is expressed in the central nervous system and bone marrow.

Function
TPO regulates the megakaryocytopoiesis and thrombopoiesis.
Interaction
Megakaryocytes, platelets, hemangioblasts, hematopoietic stem cells, and endothelial cells
Ligand/Receptor
c-Mpl
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Avanzi G, et al. 1988. Br. J. Haematol. 69:359.
  2. Deutsch V.R. and Tomer A. 2006. Br. J. Haematol. 134:453.
  3. Drachman JG, et al. 1995. J. Biol. Chem. 270:4979.
  4. Li J, et al. 1999. Br. J. Haematol. 106:345.
  5. Kato T, et al. 1997. Proc. Natl. Acad. Sci. 94:4669.
  6. Ehrenreich H, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:862.
  7. Royer Y, et al. 2005. J. Biol. Chem. 280:27251.
  8. Kaushansky K. 2005. J. Clin. Invest. 115:3339.
  9. de Graaf CA and Metcalf D. 2011. Cell Cycle. 10:1582.
  10. Zhang J, et al. 2010. J. Interferon Cytokine Res. 7:465.
  11. Lupia E, et al. 2012. Mediators Inflamm. 2012:390892.
  12. Besancenot R, et al. 2014. Blood. 124:2104.
Gene ID
7066 View all products for this Gene ID
UniProt
View information about Thrombopoietin on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/28/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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