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Ultra-LEAF™ Purified anti-human CD117 (c-kit) (Flexi-Pure™) Antibody

Pricing & Availability
Clone
104D2 (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
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Cat # Size Price Quantity Save
285767 1 mg £102
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285768 5 mg £369
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MOLM-1 megakaryocytic cell line
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 4 mg/mL and 10 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC
Recommended Usage

Our Ultra-Low Endotoxin, Azide-Free (Ultra-LEAF™) formulation is designed for in vitro and in vivo cellular and animal model applications. The antibodies are 0.2 µm filtered and do not contain any preservatives (azide free). It is recommended that the reagent be titrated for optimal performance.

Application Notes

The 104D2 antibody does not block binding of c-Kit ligand. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy1, and spatial biology (IBEX)4,5.

Additional Product Notes

Flexi-Pure™ products are rapidly-made custom purified antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
c-Kit ligand
Modification
Multiple phosphorylation sites
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

What are Flexi-Pure™ antibodies?

Flexi-Pure are rapidly made, made-to-order purified antibodies that are more concentrated than our traditional Ultra-LEAF antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Pure are the same as that of Ultra-LEAF.

How quickly will I receive my Flexi-Pure™ antibody order?

We aim to ship the Flexi-Pure antibodies within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How do I determine the optimal concentration for using my Flexi-Pure™ antibodies? How should I titrate my antibody?

Flexi-Pure antibodies are provided at concentrations between 4 - 10 mg/mL. We recommend that you titrate your antibody to determine the optimal concentration to use for your application.

Are the specifications of Ultra-LEAF™ and Flexi-Pure™ antibodies the same?

Yes, they are the same. For example, neither Ultra-LEAF, nor Flexi-Pure antibodies are tested for pathogens. In addition, they are not tested for functional applications unless indicated otherwise in their respective Technical Datasheet. However, there are differences in concentration. Typically, Flexi-Pure™ antibodies are more concentrated than the Ultra-LEAF™ antibodies.

I can’t find the Flexi-Pure™ antibody I need. When will it be available?

Please contact your local Field Applications Scientist.

I need help to validate the performance of my Flexi-Pure™ antibody. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more sizes other than 1 mg or 5 mg of Flexi-Pure™?

Yes, for bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Pure™ antibody?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-human CD117 (c-kit) 104D2 FC
APC/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 711™ anti-human CD117 (c-kit) 104D2 FC
FITC anti-human CD117 (c-kit) 104D2 FC
Alexa Fluor® 488 anti-human CD117 (c-kit) 104D2 FC,SB
Alexa Fluor® 647 anti-human CD117 (c-kit) 104D2 FC
APC/Fire™ 750 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 785™ anti-human CD117 (c-kit) 104D2 FC
PE anti-human CD117 (c-kit) 104D2 FC
APC anti-human CD117 (c-kit) 104D2 FC
TotalSeq™-A0061 anti-human CD117 (c-kit) 104D2 PG
TotalSeq™-C0061 anti-human CD117 (c-kit) 104D2 PG
TotalSeq™-B0061 anti-human CD117 (c-kit) 104D2 PG
Alexa Fluor® 700 anti-human CD117 (c-kit) 104D2 FC
APC anti-human CD117 (c-kit) 104D2 FC
PE anti-human CD117 (c-kit) 104D2 FC,SB
Purified anti-human CD117 (c-kit) 104D2 FC,CyTOF®,ICC,IP
Biotin anti-human CD117 (c-kit) 104D2 FC
PE/Cyanine5 anti-human CD117 (c-kit) 104D2 FC
PE/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
PerCP/Cyanine5.5 anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 421™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 605™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 510™ anti-human CD117 (c-kit) 104D2 FC
Brilliant Violet 650™ anti-human CD117 (c-kit) 104D2 FC
Purified anti-human CD117 (c-kit) (Maxpar® Ready) 104D2 FC,CyTOF®
Spark NIR™ 685 anti-human CD117 (c-kit) Antibody 104D2 FC
PE/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
APC/Fire™ 750 anti-human CD117 (c-kit) 104D2 FC
TotalSeq™-D0061 anti-human CD117 (c-kit) 104D2 PG
PE/Dazzle™ 594 anti-human CD117 (c-kit) 104D2 FC
PerCP/Cyanine5.5 anti-human CD117 (c-kit) 104D2 FC
GMP APC anti-human CD117 (c-kit) 104D2 FC
GMP PE anti-human CD117 (c-kit) 104D2 FC
Spark Red™ 718 anti-human CD117 (c-kit) (Flexi-Fluor™) 104D2 FC
GMP PE/Dazzle™ 594 anti-human CD117 (c-kit) 104D2 FC
GMP PE/Cyanine7 anti-human CD117 (c-kit) 104D2 FC
Pacific Blue™ anti-human CD117 (c-kit) 104D2 FC
GMP PerCP/Cyanine5.5 anti-human CD117 (c-kit) 104D2 FC
Ultra-LEAF™ Purified anti-human CD117 (c-kit) (Flexi-Pure™) 104D2 FC
Spark UV™ 387 anti-human CD117 (c-kit) (Flexi-Fluor™) 104D2 FC
Spark YG™ 581 anti-human CD117 (c-kit) (Flexi-Fluor™) 104D2 FC
Spark YG™ 593 anti-human CD117 (c-kit) (Flexi-Fluor™) 104D2 FC
Go To Top Version: 1    Revision Date: 12/24/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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