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Purified anti-CD19 Antibody

Pricing & Availability
Clone
W24107D (See other available formats)
Regulatory Status
RUO
Other Names
B-lymphocyte antigen CD19, T-cell surface antigen Leu-12, Cluster of Differentiation 19
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W24107D_PURE_CD19_IHC-P_092925
IHC staining with Purified anti-CD19 (clone W24107D), on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panels A and C) and with (panels B and D) Purified anti-CD19 (clone W24107D) followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Tissues were co-stained with Alexa Fluor® 647 anti-CD20 (Cat. No. 382804) (panel D, pseudo-colored green) and nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • a. W24107D_PURE_CD19_IHC-P_092925
    IHC staining with Purified anti-CD19 (clone W24107D), on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panels A and C) and with (panels B and D) Purified anti-CD19 (clone W24107D) followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Tissues were co-stained with Alexa Fluor® 647 anti-CD20 (Cat. No. 382804) (panel D, pseudo-colored green) and nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • b. W24107D_PURE_CD19_WB_092925
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-CD19 (clone W24107D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
648882 25 µg £100
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648883 100 µg £264
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Description

CD19 represents one of the most clinically significant and reliable biomarkers in hematologic medicine. This transmembrane glycoprotein serves as the gold standard for B cell identification and characterization throughout the entire B cell lifecycle—from early development through mature B cell stages. CD19 is a transmembrane glycoprotein that plays a central role in B cell development, activation, and signaling. It functions as a coreceptor that amplifies signaling through the B cell receptor (BCR), lowering the threshold for B cell activation. CD19 forms a complex with other surface molecules such as CD21 and CD8, enhancing calcium signaling and downstream kinase activation. It is expressed from early B cell development through mature B cells but is downregulated upon terminal differentiation into plasma cells. Because of its consistent expression on B cells and role in immune regulation, CD19 is a key target in B cell-directed immunotherapies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human CD19
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested.  For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

RRID
AB_3720624 (BioLegend Cat. No. 648882)
AB_3720624 (BioLegend Cat. No. 648883)

Antigen Details

Structure
CD19 is a 556 amino acid protein with a predicted molecular weight of 61.1 kD.
Distribution

Cell surface of B lineage cells (except plasma cells) and follicular dendritic cells

Function
Co-receptor
Interaction
Forms the classic B-cell coreceptor complex with CD21 and CD81, which associates with the core B cell receptor. Also associates with CD9 and CD82, and various intracellular signaling and adaptor proteins such as Lyn, PI3K, Vav1, and BLNK.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/29/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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