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PE anti-CD340 (ErbB2/HER-2) Antibody

Pricing & Availability
Clone
W22266D (See other available formats)
Regulatory Status
RUO
Other Names
Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W22266D_PE_CD340_ICFC_091725
SK-BR-3 cells (positive cell line, filled histogram) and HeLa cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with PE anti-CD340 (ErbB2/HER-2) (clone W22266D), or PE anti-Rat IgG2b, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) (clone RTK4530) (Cat. No. 400607).
  • W22266D_PE_CD340_ICFC_091725
    SK-BR-3 cells (positive cell line, filled histogram) and HeLa cells (negative cell line, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with PE anti-CD340 (ErbB2/HER-2) (clone W22266D), or PE anti-Rat IgG2b, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) (clone RTK4530) (Cat. No. 400607).
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639953 25 tests £113
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639954 100 tests £284
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Description

CD340, also commonly known as HER-2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two CheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand- binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in lung adenocarcinoma, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. CD340 is a therapeutic target in several human cancers and it has been shown that overexpression in breast cancers confers Taxol resistance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the intracellular domain of human CD340 (ErbB2/HER-2)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).
Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.

RRID
AB_3720621 (BioLegend Cat. No. 639953)
AB_3720621 (BioLegend Cat. No. 639954)

Antigen Details

Structure
CD340 is a 1255 amino acid protein with a predicted molecular weight of 137.9 kD. It is a glycosylated receptor-like transmembrane protein; due to glycosylation, the observed molecular weight by Western Blot is closer to 175-185 kD.
Distribution

Cellular membrane and also present in internalized vesicles

Function
Receptor, transcriptional regulator
Interaction
Homodimerizes
Heterodimerizes with EGFR, ErbB3, ErbB4, PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL
Ligand/Receptor
Heterodimerizes with other ERBB family members upon ligand binding, but does not directly bind ligand
Cell Type
Leukemia
Biology Area
Cell Biology, Immunology, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Akiyama T, et al. 1986. Science. 232:1644-6.
  2. Bargmann CI, et al. 1986. Nature. 319:226-30.
  3. Pegram MD, et al. 1997. Oncogene. 15:537-47.
  4. Slamon DJ, et al. 1989. Science. 244:707-12.
  5. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID
2064 View all products for this Gene ID
UniProt
View information about CD340 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09/17/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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