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Brilliant Violet 570™ anti-human FCGR2A/CD32A Antibody

Pricing & Availability
Clone
S20004A (See other available formats)
Regulatory Status
RUO
Other Names
FcγRIIa, FCGR2A, CD32A
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S20004A_BV570_FCGR2A_FC_042825-2
Human peripheral blood monocytes were stained with anti-human FCGR2A/CD32A (clone S20004A) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
  • S20004A_BV570_FCGR2A_FC_042825-2
    Human peripheral blood monocytes were stained with anti-human FCGR2A/CD32A (clone S20004A) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
Compare all formats See Brilliant Violet 570™ spectral data
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365507 25 tests £152
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365508 100 tests £298
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Description

CD32A (FCGR2A) is a 40 kD, type I protein with 2 C2-set domains the extracellular region and 1 ITAM in the intracellular region. CD32A binds monomeric IgG with low affinity but is very efficient binding immune complexes.  FCGR2A is expressed by myeloid cells such as macrophages, monocytes, neutrophils, eosinophils and dendritic cells; CD32a is also expressed by epithelial cells, platelets and activated CD4+ T cells. CD32a is involved in phagocytosis and clearing of immune complexes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FCGR2A-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

 This clone does not recognizes CD32B-transfected cell

RRID
AB_3716969 (BioLegend Cat. No. 365507)

Antigen Details

Structure
Member of the Ig superfamily, 40 kD, type I protein, 2 C2-set domains, 1 ITAM
Distribution

Macrophages, monocytes, neutrophils, eosinophil, platelets, and CD4+ T Cells

Function
Phagocytosis, cytotoxicity, platelet aggregation
Ligand/Receptor
Aggregated IgG, immune complexes
Cell Type
Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets
Biology Area
Adaptive Immunity, Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Hubbard J. et al. 2020. J Exp Med. 217:e20200359
  2. Newling M, et al. 2020. Clin Exp Immunol. 199:39-49
  3. Maria H, et al. 2018. Front Immunol. 9:2814
  4. Descours B, et al. 2017. Nature. 543:564-567
Gene ID
NA
UniProt
View information about FCGR2A/CD32A on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/28/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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