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Brilliant Violet 421™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Brilliant Violet 421™
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Product Citations
publications
SAV_BV421_071911
Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
  • SAV_BV421_071911
    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with SAV-Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
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405226 100 µL £133
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405225 100 µg £317
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 421™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 421™ by 405 nm laser light induces a fluorescence maximum emission of 421 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Cat. No. 405225: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Cat. No. 405226: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
Streptavidin was conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
405225 is bottled at 0.5 mg/ml.
405226 is bottled at 0.1 mg/ml.
(concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested
ICC, IHC-F, IHC-P
Recommended Usage

Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 421™ conjugation. For immunofluorescent staining, we recommend using Cat. No. 405226 at dilutions ≤ 0.015 µg in 100 µL staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using Cat. No. 405225. It is recommended that the reagent be titrated for optimal performance for each application.

* Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Brilliant Violet 421™ is 340 kD and Streptavidin alone is 52 kD.

Additional Product Notes

View more applications data using this product in immunofluorescence microscopy and labeling tetramers in flow cytometry in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim G, et al. 2013. J. Immunol. 190:1510. PubMed.
  2. Giltiay NV, Et al. 2013. J. Immunol. 191:2155. PubMed
  3. Sugiyama D, et al. 2013. PNAS. 110:17945. PubMed
  4. Scally SW, et al. 2013. J. Exp Med. 210:2569. PubMed
  5. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  6. Higdon LE, et al. 2014. PNAS. 111:5652. PubMed
  7. Friedman RS, et al. 2014. PNAS. 111:9223. PubMed
  8. van de Weijer ML, et al. 2014. Nat Commun. 5:3832. PubMed
  9. Mise-Omata S, et al. 2014. Int Immunol. PubMed
  10. Choo JA, et al. 2014. J Virol. 88:10613. PubMed
  11. Xing YL, et al. 2014. J Neurosci. 34:14128. PubMed
  12. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  13. Poh Cm, et al. 2014. Infect Immun. 82:4854. PubMed
  14. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  15. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  16. Krishnaswamy JK, et al. 2015. PNAS. 112:3056. PubMed
  17. Rampuria P, et al. 2015. Int Immunol. 27:253. PubMed
Product Citations
  1. Takizawa F, et al. 2016. J Immunol. 196: 4522 - 4535. PubMed
  2. Steines L, et al. 2021. Front Immunol. 12:657894. PubMed
  3. García-Alegria E, et al. 2021. STAR Protoc. 2:100367. PubMed
  4. Reyes RA, et al. 2021. PLoS One. 16:e0261656. PubMed
  5. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  6. He T, et al. 2022. Liver Int. 42:1287. PubMed
  7. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  8. Wolf EW, et al. 2022. Proc Natl Acad Sci U S A. 119:e2201794119. PubMed
  9. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  10. Lei X, et al. 2022. J Exp Med. 219: . PubMed
  11. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  12. Wang Z, et al. 2022. J Exp Med. 219: . PubMed
  13. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  14. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  15. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
  16. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  17. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  18. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  19. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  20. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  21. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  22. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  23. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  24. Sun P, et al. 2022. iScience. 25:105202. PubMed
  25. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  26. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
  27. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
  28. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
  29. Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed
  30. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  31. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  32. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  33. Fadzeyeva E, et al. 2023. iScience. 26:106748. PubMed
  34. Sigmund EC, et al. 2023. PLoS One. 18:e0285597. PubMed
  35. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  36. Angkasekwinai P, et al. 2017. PLoS One. 10.1371/journal.pone.0184684. PubMed
  37. van Eeden C, et al. 2018. Cell Rep. 25:3123. PubMed
  38. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  39. Hayashi M, et al. 2017. EBioMedicine. 15:127-136. PubMed
  40. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  41. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  42. Chaugule S, et al. 2021. Int J Mol Sci. 22:. PubMed
  43. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  44. Parra–Sánchez H, et al. 2019. Viruses. 1.153472222. PubMed
  45. Miura M, et al. 2019. Wellcome Open Res. 3:105. PubMed
  46. Xing Y, et al. 2014. J Neurosci. 34:14128. PubMed
  47. Sei J, et al. 2016. PLoS One. 11: 0152192. PubMed
  48. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  49. Sage P, et al. 2016. Nat Immunol. 17:1436-1446. PubMed
  50. Xiong W, et al. 2021. Int J Mol Sci. 22:. PubMed
  51. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  52. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  53. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  54. Trivedi S, et al. 2015. Vaccine. 33: 7315-7327. PubMed
  55. Sugiyama D, et al. 2013. Proc Natl Acad Sci U S A. 110:17945. PubMed
  56. Giltiay N, et al. 2013. J Immunol. 191:2155. PubMed
  57. Poon MML, et al. 2021. Sci Immunol. 6:eabl9105. PubMed
  58. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  59. Mitra T, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.06.035. PubMed
  60. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  61. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  62. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  63. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  64. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  65. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  66. Im S, et al. 2016. Nature. 537:417-421. PubMed
  67. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  68. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  69. Gaudette BT, et al. 2021. J Clin Invest. 131:. PubMed
  70. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  71. Koay H, et al. 2016. Nat Immunol. 10.1038/ni.3565. PubMed
  72. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  73. Zhang R, et al. 2021. MAbs. 13:1904546. PubMed
  74. Neumann B, et al. 2019. Cell Stem Cell. 25:473. PubMed
  75. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  76. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  77. Krishnaswamy J, et al. 2015. Proc Natl Acad Sci U S A. 112:3056. PubMed
  78. Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed
  79. Rowan A, et al. 2016. PLoS Pathog. 12:e1006030. PubMed
  80. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  81. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  82. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  83. Bähr A, et al. 2016. PLoS One. 11: 0155676. PubMed
  84. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  85. Liu B, et al. 2015. J Immunol. 194:3583. PubMed
  86. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  87. Tarke A, et al. 2022. Cell. 185:847. PubMed
  88. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  89. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  90. Cavazzoni CB, et al. 2022. Cell Rep. 38:110399. PubMed
  91. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  92. Elayeb R, et al. 2016. Haematologica. 101: 209 - 218. PubMed
  93. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  94. Weijer M, et al. 2014. Nat Commun. 5:3832. PubMed
  95. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  96. Sanjo H, et al. 2015. PLoS One. 10: 0128761. PubMed
  97. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  98. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  99. Borroto A, et al. 2022. Front Immunol. 13:799919. PubMed
  100. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  101. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  102. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  103. Yang E, et al. 2016. J Immunol. 197: 934 - 941. PubMed
  104. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  105. Tsuzuki S, et al. 2015. Int Immunol. 10.1093/intimm/dxv058. PubMed
  106. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  107. Scally S, et al. 2013. J Exp Med. 210:2569. PubMed
  108. Blomberg R, et al. 2019. Matrix Biol. 83:60. PubMed
  109. Claudepierre M, et al. 2014. J Virol. 88:5242. PubMed
  110. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  111. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  112. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
  113. Wang Z, et al. 2022. J Exp Med. 219:. PubMed
  114. Guelen L, et al. 2022. J Immunother Cancer. 10:. PubMed
  115. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  116. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  117. Dan JM, et al. 2021. Science. :371. PubMed
  118. Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
  119. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  120. Lederer K, et al. 2022. Cell. . PubMed
  121. Müller TR, et al. 2021. Cell Rep Med. 2:100374. PubMed
  122. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  123. Lang P 2015. Int Immunol. 27:253. PubMed
  124. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  125. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  126. Zhu L, et al. 2016. J Cell Sci. 129: 4238 - 4251. PubMed
  127. Fries CN, et al. 2021. Sci Rep. 11:14494. PubMed
  128. An X, et al. 2017. PLoS One.. 10.1371/journal.pone.0181904. PubMed
  129. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  130. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  131. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  132. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  133. Wells DK, et al. 2020. Cell. 183:818. PubMed
  134. Clement RL, et al. 2019. Nat Immunol. 20:1360. PubMed
  135. Kim JM, et al. 2020. Nat Commun. 11:2289. PubMed
  136. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  137. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  138. Choo J, et al. 2014. J Virol. 88:10613. PubMed
  139. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  140. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  141. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  142. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  143. Higdon L, et al. 2014. Proc Natl Acad Sci U S A. 111:5652. PubMed
  144. Kim G, et al. 2013. J Immunol. 190:1510. PubMed
  145. Cho A, et al. 2022. J Exp Med. 219:. PubMed
  146. Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed
  147. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed
  148. Binnewies M, et al. 2019. Cell. 177:556. PubMed

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 8    Revision Date: 12/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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