StarBright UltraViolet 740 anti-human CD279 (PD-1) antibody, CD279, EH12.2H7

Pricing & Availability

Clone: EH12.2H7 (See other available formats)
Regulatory Status: RUO
Other Names: PD-1, PDCD1
Isotype: Mouse IgG1, κ
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Product Citations: publications

a.
EH122H7_CD279_FC_1_070925 — Human peripheral blood cells were stained with anti-human CD3 (clone UCHT1) APC and anti-human CD279 (PD-1) (clone EH12.2H7) StarBright UltraViolet 740 (left) or stained with anti-human CD3 (clone UCHT1) APC only (right). Data shown are gated on the lymphocyte population.

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329984

100 tests

DKK2228

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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.

StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections¹³, and spatial biology (IBEX)^15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Antigen Details

Structure: Immunoglobulin superfamily
Distribution: Transiently expressed on CD4^- CD8^- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells
Ligand/Receptor: B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Gene ID: 5133 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What is a “StarBright panel”?: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Will my StarBright panel be provided as a cocktail?: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Are StarBright GMP/ASR products available?: StarBright ASR and GMP products are coming soon.
Are StarBright custom products available?: Yes, please contact our Custom Solutions Team.
Will StarBright isotype controls be offered?: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
What amount of a StarBright conjugate should I use?: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD279 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD279 (PD-1) (Maxpar® Ready)	EH12.2H7	FC,CyTOF®
GoInVivo™ Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC
Brilliant Violet 650™ anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 700 anti-human CD279 (PD-1)	EH12.2H7	FC
APC/Fire™ 750 anti-human CD279 (PD-1)	EH12.2H7	FC
TotalSeq™-A0088 anti-human CD279 (PD-1)	EH12.2H7	PG
TotalSeq™-B0088 anti-human CD279 (PD-1)	EH12.2H7	PG
TotalSeq™-C0088 anti-human CD279 (PD-1)	EH12.2H7	PG
Brilliant Violet 750™ anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 421™ anti-human CD279 (PD-1)	EH12.2H7	FC
FITC anti-human CD279 (PD-1)	EH12.2H7	FC
PE anti-human CD279 (PD-1)	EH12.2H7	FC,SB
APC anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 647 anti-human CD279 (PD-1)	EH12.2H7	FC
Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC-F
PerCP/Cyanine5.5 anti-human CD279 (PD-1)	EH12.2H7	FC
APC/Cyanine7 anti-human CD279 (PD-1)	EH12.2H7	FC
Pacific Blue™ anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Cyanine7 anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 605™ anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 711™ anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 785™ anti-human CD279 (PD-1)	EH12.2H7	FC
Ultra-LEAF™ Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC-F
Brilliant Violet 510™ anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Dazzle™ 594 anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 488 anti-human CD279 (PD-1)	EH12.2H7	FC
PerCP anti-human CD279 (PD-1)	EH12.2H7	FC
Biotin anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Fire™ 640 anti-human CD279 (PD-1)	EH12.2H7	FC
TotalSeq™-D0088 anti-human CD279 (PD-1)	EH12.2H7	PG
PE/Cyanine5 anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Fire™ 744 anti-human CD279 (PD-1)	EH12.2H7	FC
Spark Red™ 718 anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 570™ anti-human CD279 (PD-1)	EH12.2H7	FC
Spark NIR™ 685 anti-human CD279 (PD-1) Antibody	EH12.2H7	FC
Spark PLUS B550™ anti-human CD279 (PD-1) Antibody	EH12.2H7	FC
Spark YG™ 581 anti-human CD279 (PD-1) (Flexi-Fluor™)	EH12.2H7	FC
Spark YG™ 593 anti-human CD279 (PD-1) (Flexi-Fluor™) Antibody	EH12.2H7	FC
StarBright UltraViolet 740 anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Fire™ 700 anti-human CD279 (PD-1) Antibody	EH12.2H7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07/22/2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.