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Purified anti-HMGA2 Antibody

Pricing & Availability
Clone
W23012E (See other available formats)
Regulatory Status
RUO
Other Names
High Mobility Group AT-Hook 2, High-Mobility Group (Nonhistone Chromosomal) Protein
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. W23012E_PURE_HMGA2_WB_073125A
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel. A549 cells were untreated or treated with human TGF-β1 (10 ng/mL) (Cat. No. 781804) and human IL-1β (5 ng/mL) (Cat. No. 579404) for 48 hours. Then, the gel was transferred to a PVDF membrane, and probed with the Purified anti-HMGA2 (clone W23012E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W23012E_PURE_HMGA2_WB_073125A
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel. A549 cells were untreated or treated with human TGF-β1 (10 ng/mL) (Cat. No. 781804) and human IL-1β (5 ng/mL) (Cat. No. 579404) for 48 hours. Then, the gel was transferred to a PVDF membrane, and probed with the Purified anti-HMGA2 (clone W23012E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W23012E_PURE_HMGA2_WB_2_073125A
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the Purified anti-HMGA2 (clone W23012E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:5000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • C. W23012E_PURE_HMGA2_ICC_073125
    A549 cells were untreated (mock) and treated with human TGF-β1 (10 ng/mL) (Cat. No. 781804) and human IL-1β (5 ng/mL) (Cat. No. 579404) for 48 hours. The mock and treated A549 cells were fixed with Fixation buffer (Cat. No. 420801) for 10 minutes, permeabilized with TritonX-100 then blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with Purified Rat IgG2b, κ Isotype Control Antibody (Cat. No. 400602) (panels A, D) or stained with Purified anti- HMGA2 (clone W23012E) (panels B, E), followed with Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) and actin cytoskeleton were labeled by Flash Phalloidin™ Red 594 (Cat. No. 424203). The image was captured with a 40X objective. Scale bar: 25 µm
  • D. W23012E_PURE_HMGA2_IHC-P_1_072425
    IHC staining with Purified anti-HMGA2 (clone W23012E) was performed on FFPE human testis tissue following antigen retrieval using 1X Citrate buffer (pH 6.0) (Cat. No. 420902). The tissue sections were incubated with Purified anti-HMGA2 (clone W23012E) (panel B and merged in panel C) followed by Alexa Fluor® 594 Goat anti-rat (Cat. No. 405422) (red) or secondary antibody only (negative control, panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) (panels A and C). The image was captured with a 40X objective. Scale Bar: 50 µm
  • E. W23012E_PURE_HMGA2_IHC-P_2_072425
    IHC staining with Purified anti-HMGA2 (clone W23012E) was performed on FFPE human colon cancer following antigen retrieval using 1X Citrate buffer (pH 6.0) (Cat. No. 420902). The tissue sections were incubated with Purified anti-HMGA2 (clone W23012E) (panel B and merged in panel C) followed by Alexa Fluor® 594 Goat anti-rat (Cat. No. 405422) (red) or secondary antibody only (negative control, panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (blue) (panels A and C). The image was captured with a 40X objective. Scale Bar: 50 µm
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648836 25 µg DKK1040
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648837 100 µg DKK2673
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Description

HMGA2, also known as the high-mobility group AT-hook 2, is a nonhistone transcriptional regulator belonging to the high mobility group (HMG) protein family with AT-hook DNA binding motif. HMGA2 is highly expressed during early mammalian embryogenesis but is hardly detected in normal adult tissues. Recently, it was discovered that HMGA2 is upregulated in a variety of tumors including gastric cancer, ovarian cancer, non-small cell lung cancer and colorectal cancer, suggesting that it has a role in controlling tumor progression. The cancers with overexpression HMGA2 have a significantly stronger response to double strand breaks in DNA caused by radiation therapy and certain chemotherapy treatments. HMGA2 also has been implicated in diet-induced obesity in the gene knockout study of the mouse.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human HMGA2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

The clone cross-reacts with mouse and rat in western blot (WB). 

For immunocytochemistry (ICC), we recommend 4% PFA fixation (Cat. No. 420801) followed by permeabilization with Triton X-100 or ice-cold methanol.

For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) are recommended.

RRID
AB_3720546 (BioLegend Cat. No. 648836)
AB_3720546 (BioLegend Cat. No. 648837)

Antigen Details

Structure
HMGA2 is a 109 amino acid protein with a predicted molecular weight of 12 kD.
Distribution

Nucleus. Expressed predominantly during embryogenesis.

Function
Transcriptional regulator, cell cycle regulation.
Interaction
E4F1, NEK2
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Stem Cells
Antigen References
  1. Cleynen I, et al. 2008. Int J Oncol. 32:289.
  2. Monzen K, et al. 2008. Nat Cell Biol. 10:567.
  3. Fusco A and Fedele M. 2007. Nat Rev Cancer. 7:899.
  4. Rawlinson N.J, et al. 2008. Cancer Genet Cytogenet.181(2):119.
  5. Wei J.J, et al. 2010. Am J Surg Pathol. 34(1):18.
  6. Mahajan,A, et al. 2010. Mod Pathol. 23(5),673.
  7. Ming- Boo L, et al. 2005. Cancer Res. 65(15):6622.
  8. Zhou X, et al. 1995. Nature. 376(6543):771.
  9. Anand A, et al. 2000. Nature Genetics. 24:377.
Gene ID
8091 View all products for this Gene ID
UniProt
View information about HMGA2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/31/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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