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PE anti-mouse AhR Antibody

Pricing & Availability
Clone
W16012C (See other available formats)
Regulatory Status
RUO
Other Names
bHLHe76, aryl hydrocarbon receptor, Aryl hydrocarbon receptor, RP85
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16012C_PE_AhR_Antibody_072825
C57BL/6 mouse splenocytes were stimulated with (left) or without (right) recombinant human TGF-β1 and recombinant mouse IL-6 for 3 days. Cells were surface stained with anti-mouse CD4 APC, fixed and permeabilized, then intracellularly stained with anti-mouse AhR PE (clone W16012C).
  • W16012C_PE_AhR_Antibody_072825
    C57BL/6 mouse splenocytes were stimulated with (left) or without (right) recombinant human TGF-β1 and recombinant mouse IL-6 for 3 days. Cells were surface stained with anti-mouse CD4 APC, fixed and permeabilized, then intracellularly stained with anti-mouse AhR PE (clone W16012C).
Compare all formats See PE spectral data
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162703 25 µg DKK1272
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Description

The aryl hydrocarbon receptor (AhR) is a basic helix-loop-helix transcription factor that is activated by multiple ligands such as TCDD. It is expressed in many immune cells and performs an important role in immune regulation. AhR is induced under Th17-polarizing conditions. In its inactive state, AhR is present in the cytoplasm, but upon ligand binding, it translocates to the nucleus. Activation of AhR is tightly controlled by ubiquitination, ligand metabolism, and AhR complex disruption.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2892335 (BioLegend Cat. No. 162703)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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