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Biotinylated Recombinant Human MUC16 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CA125, CA-125, MUC-16, CA125 ovarian cancer antigen, cancerantigen 125
Ave. Rating
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Product Citations
publications
a. Biotin-Recom_Human-MUC16_1_CF
Recombinant human MUC16 binds to immobilized recombinant human Mesothelin-Fc chimera (Cat. No. 593202) at 0.5 µg/mL in a dose-dependent manner. The ED50 for this effect is 0.07 – 0.42 ng/mL.
  • a. Biotin-Recom_Human-MUC16_1_CF
    Recombinant human MUC16 binds to immobilized recombinant human Mesothelin-Fc chimera (Cat. No. 593202) at 0.5 µg/mL in a dose-dependent manner. The ED50 for this effect is 0.07 – 0.42 ng/mL.
  • b. Biotin-Recom_Human-MUC16_2_CF
    Stability Testing for Biotinylated Recombinant Human MUC16. Biotinylated recombinant human MUC16 lyophilized was kept at -20°C (Control), another sampler was reconstituted in PBS pH 7.2 and kept at 4°C for two weeks (2w 4°C). The samples were tested by their property to bind to immobilized recombinant human Mesothelin-Fc chimera (Cat. No. 593202) at 0.5 µg/mL in a dose-dependent manner. The ED50 for this effect is 0.07 – 0.42 ng/mL.
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717755 50 µg DKK1159
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Description

MUC16 was initially identified as CA125 which is a marker for ovarian cancer. MUC16 is a type I transmembrane mucin and contains 22,152 amino acids. MUC16 possesses three domains: the amino terminal (serine/threonine rich domain), the tandem domain, and the carboxy terminal domain. The latter consist of an extracellular part, transmembrane, and a short intracellular tail. On shearing, MUC16 becomes CA125. MUC16 is expressed in normal epithelial with an essential function of protection and lubrication. MUC-16 has been associated to multiple cancers such as ovarian cancer, pancreatic cancer, endometrial carcinoma, interstitial lung disease, and acute heart failure. MUC16 induces cell proliferation by regulation of glucose transporter 1 (GLUT1) expression in epithelial ovarian cancer (EOC). CA125 and mesothelin are co-expressed in advanced grade ovarian adenocarcinoma. It has been speculated that CA125 might contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to mesothelin. Also, mesothelin and CA125/mucin 16 (CA125/MUC16) are co-overexpressed at the invading edges of pancreatic cancer tissues, and their expression correlates with poor survival rates.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human MUC16, amino acids (Met13360 – Gln14347) (Accession# Q8WXI7), was expressed in CHO cells. The carboxy terminus contains 6His-Avi tag. Human MUC16-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 1014 amino acid recombinant protein has a predicted molecular mass of approximately 112.5 kD. The protein migrates at approximately 140 - 170 kD in DTT-reducing and non-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
Lyophilized from 0.22 µm filtered protein solution in PBS pH7.2, 5% trehalose.
Endotoxin Level
Less than 0.1 EU per µg of cytokine as determined by the LAL method.
Concentration
50 µg size lyophilized
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C or colder until the expiration date. Reconstitute lyophilized protein in PBS pH7.2. Before reconstitution, make sure sterile PBS pH7.2 and product are at room temperature. Quickly spin the vial or gently tap down on the vial to make sure the lyophilized product is at the bottom of the vial before opening. Use aseptic techniques to add the required volume of reconstitution buffer (sterile PBS pH7.2) to the vial, to obtain the recommended stock concentration 250 µg/mL. Close the vial and leave at ambient temperature for 15-20 minutes. Then gently invert the vial several times or until all the lyophilized product dissolves. Leave the vial at room temperature for another 15 minutes. If small particulates are still observed after 15 minutes, incubate at room temperature for an additional 30 minutes, and leave the vial at 2°C - 8°C overnight. Next day, invert the vial several times or gently pipette the solution up and down before use. If needed, transfer the reconstituted stock solution to a sterile container for additional dilution to no less than 100 µg/mL. Small working aliquots in polypropylene tubes can be made after reconstitution and store the vials at -20°C or lower. Avoid freeze/thaw cycles. Carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to two weeks or stored at -20°C or colder for up to 3 months.
Activity
Recombinant human MUC16 binds to immobilized recombinant human Mesothelin-Fc chimera (Cat. No. 593202) in a dose-dependent manner. The ED50 for this effect is 0.07 – 0.42 ng/mL.
Application

Bioassay
Application Notes

Our lyophilized proteins are validated in-house to maintain activity after shipping at ambient temperature and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

Epithelial cells, limbal epithelium (corneal epithelial cells), endometrial epithelial cells. Multiple cancers, epithelial ovarian cancer, pancreatic cancer, endometrial carcinoma, esophageal, gastric, and colorectal adenocarcinomas. Interstitial lung disease.

Function
Maintain mucosal integrity and provide lubrication, protective barrier against pathogens in the limbal epithelium.
Interaction
Mesothelial cells, ovarian tumors, mesotheliomas
Ligand/Receptor
Mesothelin, Galectin 1
Bioactivity
MUC16 binds to immobilized mesothelin in a dose dependent manner.
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology
Molecular Family
Adhesion Molecules
Antigen References
  1. Yin BW and Lloyd KO. 2001. J Biol Chem. 276:27371.
  2. Streppel MM, et al. 2012. Hum Pathol. 43:1755.
  3. Gubbels JAA, et al, 2006. Mol Cancer. 5:50.
  4. Seelenmeyer C, et al, 2003. J Cell Sci. 116:1305.
  5. Wang F, et al, 2021. J Cell Mol Med. 25:3031.
  6. Zhang XJ, et al, 2024. Clin Exp Med 24:101.
Gene ID
94025 View all products for this Gene ID
UniProt
View information about MUC16 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/17/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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