Ultra-LEAF™ Purified anti-human CD47 Antibody, CD47, W24220J

Pricing & Availability

Clone: W24220J (See other available formats)
Regulatory Status: RUO
Other Names: Rh-associated protein, gp42, integrin-associated protein, IAP, neurophilin
Isotype: Rat IgG2a, κ
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Product Citations: publications

W24220J_ULEAF_CD47_Block_012626 — Biotinylated recombinant human SIRPα-Fc chimera (Cat. No. 561014; black circles) binds to immobilized recombinant human CD47. Ultra-LEAF™ Purified anti-human CD47 (clone W24220J, purple squares) inhibits the binding in a dose-response manner, whereas Ultra-LEAF™ Purified rat IgG2a, κ Isotype Control (Cat. No. 400543, gray triangles) has no inhibitory effect. This antibody blocks the binding of 500 ng/mL biotinylated recombinant human SIRPα-Fc Chimera to 1.0 µg/mL of immobilized recombinant human CD47. The ND₅₀ range to observe this effect is: 28.0 – 285.0 ng/mL. Main competitor ND₅₀ = 351.1 ng/mL.

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648719

100 µg

88€

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648720

1 mg

120€

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Description

CD47, also known as Rh-associated protein, gp42, integrin-associated protein (IAP) and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described for the cytoplasmic tail: a shorter form, expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts; and a longer form, expressed mainly in neural tissues. CD47 expression is widely distributed in hematopoietic cells, including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and a thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47, mice present defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human CD47
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality-controlled tested by blocking the binding of 1 μg/mL immobilized recombinant human CD47 (Cat. No. 785406) to its ligand biotinylated recombinant human SIRPα-Fc Chimera (Cat. No. 561016) at 500 ng /mL. ND₅₀ range: 28.0 – 285.0 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure: Integral membrane protein with an extracellular N-terminal IgV domain, five transmembrane segments, and a short C-terminal cytoplasmic tail
Distribution: Hematopoietic cells, such as thymocytes, T cells, B cells, platelets, and erythrocytes, epithelial cells, endothelial cells, fibroblasts, and neural tissues
Function: Adhesion molecule and thrombospondin receptor
Interaction: Immune cells, mainly macrophages, dendritic cells, neutrophils, T cells, NK cells, B cells, and hematopoietic stem cells; red blood cells; platelets; endothelial cells; vascular smooth muscle cells; neurons/microglia; epithelial cells; fibroblasts; and pancreatic cells
Ligand/Receptor: β3 integrins CD51/CD61, CD41/CD61
Cell Type: B cells, Endothelial cells, Epithelial cells, Erythrocytes, Fibroblasts, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Majeti R, et al. 2009. Cell. 138:286-99.

Chao MP, et al. 2010. Cell. 142:699-713.

Willingham SB, et al. 2012. Proc Natl Acad Sci USA. 109:6662-7.

Maute R, et al. 2022. Immunooncol Technol. 13:100070.

Jiang X, et al. 2021. J Hematol Oncol. 14:180.
Gene ID: 961 View all products for this Gene ID
UniProt: View information about CD47 on UniProt.org

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Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD47 Reagents Request Custom Conjugation

Description	Clone	Applications
Ultra-LEAF™ Purified anti-human CD47	W24220J	Block

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01.26.2026

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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