Loading...

StarBright UltraViolet 740 anti-human Ki-67 Antibody

Pricing & Availability
Clone
Ki-67 (See other available formats)
Regulatory Status
RUO
Other Names
Antigen Ki-67
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A. Ki-67_UCHT1_SBUV740_Ki-67_BasicPanel_071425
PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed and permeabilized with 70% ethanol. Cells were then stained with anti-human CD4 (clone RPA-T4) APC and anti-human Ki-67 (clone Ki-67) StarBright UltraViolet 740 (left) or stained with anti-human CD4 (clone RPA-T4) APC only (right). Data shown are gated on the lymphocyte population.
  • A. Ki-67_UCHT1_SBUV740_Ki-67_BasicPanel_071425
    PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed and permeabilized with 70% ethanol. Cells were then stained with anti-human CD4 (clone RPA-T4) APC and anti-human Ki-67 (clone Ki-67) StarBright UltraViolet 740 (left) or stained with anti-human CD4 (clone RPA-T4) APC only (right). Data shown are gated on the lymphocyte population.
Compare all formats
Cat # Size Price Quantity Check Availability Save
350542 100 tests 300€
Check Availability


Need larger quantities of this item?
Request Bulk Quote

This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. The antigen Ki-67 interacts with Hklp2, hNIFK, and chromobox protein homolog 1, 3, and 5. Ki-67 is required for cell proliferation and its expression is restricted to the phases G1, S, G2, and M of the cell cycle. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Formulation
Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 740 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 740 has a maximum excitation of 343 nm and a maximum emission of 737 nm.


StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.

Ki-67 Staining Protocol:

1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.
7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
  2. Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
  3. Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
  4. Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
  5. Guha P, et al. 2013. PNAS. 110:5052. PubMed

Antigen Details

Structure
Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
Distribution

Expressed in the phases G1, S, G2, and M of the cell cycle

Function
Required for cell proliferation
Interaction
Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family
Nuclear Markers
Antigen References

1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
3. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
4288 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What is a “StarBright panel”?

A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.

Will my StarBright panel be provided as a cocktail?

StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.

Are StarBright GMP/ASR products available?

StarBright ASR and GMP products are coming soon.

Are StarBright custom products available?

Yes, please contact our Custom Solutions Team.

Will StarBright isotype controls be offered?

We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.

What amount of a StarBright conjugate should I use?

Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.

Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?

For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Go To Top Version: 1    Revision Date: 06.05.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account