Spark YG™ 581 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™) Antibody, Ly-6A/E, D7

Pricing & Availability

Clone: D7 (See other available formats)
Regulatory Status: RUO
Other Names: Sca-1
Isotype: Rat IgG2a, κ
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285544

50 µg

269€

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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-dependent mouse T-cell line (CTL-L)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 581 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

* Spark YG™ 581 has a maximum excitation of 562 nm and a maximum emission of 581 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting^1,2, immunoprecipitation¹, in vitro lymphocyte activation^3-6, induction of redirected lysis⁷, induction of T cell inhibitory signalling⁸, immunofluorescence⁹, and immunohistochemical staining of acetone-fixed frozen sections¹³ and Bouin-fixed, paraffin-embedded samples⁹.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.¹⁴
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3720697 (BioLegend Cat. No. 285544)

Antigen Details

Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Cell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.
Gene ID: 110454 View all products for this Gene ID
UniProt: View information about Ly-6A/E on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

What if my requested antibody-dye combination doesn't perform as expected?

Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.

Other Formats

View All Ly-6A/E Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Ly-6A/E (Sca-1) (Maxpar® Ready)	D7	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Brilliant Violet 785™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Brilliant Violet 650™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1)	D7	FC
TotalSeq™-A0130 anti-mouse Ly-6A/E (Sca-1)	D7	PG
TotalSeq™-B0130 anti-mouse Ly-6A/E (Sca-1)	D7	PG
TotalSeq™-C0130 anti-mouse Ly-6A/E (Sca-1)	D7	PG
APC anti-mouse Ly-6A/E (Sca-1)	D7	FC
Biotin anti-mouse Ly-6A/E (Sca-1)	D7	FC,IHC
FITC anti-mouse Ly-6A/E (Sca-1)	D7	FC
PE anti-mouse Ly-6A/E (Sca-1)	D7	FC
PE/Cyanine5 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Purified anti-mouse Ly-6A/E (Sca-1)	D7	FC,IHC,IP,WB
PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Alexa Fluor® 488 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Alexa Fluor® 647 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Pacific Blue™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
Brilliant Violet 421™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
PerCP anti-mouse Ly-6A/E (Sca-1)	D7	FC
PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1)	D7	FC
APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Brilliant Violet 510™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
Brilliant Violet 711™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
Brilliant Violet 605™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
Spark NIR™ 685 anti-mouse Ly-6A/E (Sca-1)	D7	FC
Spark PLUS UV395™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
PerCP/Fire™ 806 anti-mouse Ly-6A/E (Sca-1) Antibody	D7	FC
Brilliant Violet 750™ anti-mouse Ly-6A/E (Sca-1)	D7	FC
Spark UV™ 387 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™)	D7	FC
Spark YG™ 581 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™)	D7	FC
Spark YG™ 593 anti-mouse Ly-6A/E (Sca-1) (Flexi-Fluor™)	D7	FC
PE/Fire™ 744 anti-mouse Ly-6A/E (Sca-1) Antibody	D7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 10.06.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.