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Purified anti-ZO-1 Antibody

Pricing & Availability
Clone
W24004C (See other available formats)
Regulatory Status
RUO
Other Names
tight junction protein 1 (TJP1), zona occludens protein 1 (ZO1)
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
A. W24004C_PURE_ZO-1_IHC-P_091725
IHC staining with Purified anti-ZO-1 (clone W24004C), on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panels A and C) and with (panel B and D) Purified anti-ZO-1 (clone W24004C) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801, merged in panels A and B). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • A. W24004C_PURE_ZO-1_IHC-P_091725
    IHC staining with Purified anti-ZO-1 (clone W24004C), on formalin-fixed paraffin-embedded human colon tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panels A and C) and with (panel B and D) Purified anti-ZO-1 (clone W24004C) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801, merged in panels A and B). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • B. W24004C_PURE_ZO-1_ICC_091725
    HAP1 ZO-1 Knockout cell line, (Horizon Cat. No. HZGHC006271c003, panel A) and HAP1 WT cells (high-expressing cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified ZO-1 (clone W24004C), followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801, panels C & D). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. W24004C_PURE_ZO-1_WB_091725
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-ZO-1 (clone W24004C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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648874 25 µg 136€
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648875 100 µg 352€
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Description

Zonula occludens-1 (ZO-1) is a scaffolding protein critical for the assembly and maintenance of tight junctions in epithelial and endothelial cells. It links transmembrane tight junction proteins such as claudins and occludin to the actin cytoskeleton, thereby maintaining cell polarity and barrier integrity. ZO-1 also participates in signal transduction pathways that regulate cell proliferation and differentiation. Loss or mislocalization of ZO-1 is associated with impaired barrier function and has been implicated in various pathological conditions, including cancer and inflammatory diseases.

Product Details
Quality Statement

This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: Human HAP1 Knockout Cell Line – TJP1 (Horizon Cat. No. HZGHC006271c003)

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein corresponding to a central portion of human ZO-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

RRID
AB_3720606 (BioLegend Cat. No. 648874)
AB_3720606 (BioLegend Cat. No. 648875)

Antigen Details

Structure
ZO-1 is a 1748 amino acid protein with a predicted molecular weight of 195.5 kD.
Distribution

Cell surface, tight junctions

Function
Scaffold protein
Interaction
Claudins (CLDN1-5), Occludin (OCLN), actin
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Adhesion, Signal Transduction
Molecular Family
Adhesion Molecules, Cytoskeletal Proteins
Antigen References
  1. Kuo W, et al. 2022. Ann N Y Acad Sci. 1514(1):21-33.
  2. Van Itallie CM, et al. 2009. Mol Biol Cell. 20(17):3930-3940.
  3. Tornavaca O, et al. 2015. J Cell Biol. 208(6):821-838. 
  4. Stevenson BR, et al. 1986. J Cell Biol. 103(3):755-66.
Gene ID
7082 View all products for this Gene ID
UniProt
View information about ZO-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.17.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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