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Purified anti-VILIP-1 Antibody

Pricing & Availability
Clone
A22106J (See other available formats)
Regulatory Status
RUO
Other Names
Visinin-like protein 1, VILIP, VILIP1, VLP-1, VLP1, VSNL1, VISL1, Hippocalcin-like protein 3 , HLP3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A22106J_PURE_VILIP-1_WB_1_072525
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-VILIP-1 (clone A22106J). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. A22106J_PURE_VILIP-1_WB_1_072525
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-VILIP-1 (clone A22106J). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. A22106J_PURE_VILIP-1_WB_072525
    IHC staining with Purified anti-VILIP-1 (clone A22106J) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-VILIP-1 (clone A22106J) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a 40X objective and merged (panel C and D). Scale bar: 50 µm
  • C. A22106J_PURE_VILIP-1_WB_072525
    Whole cell extracts (15 µg total protein per lane) from indicated tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-VILIP-1 (clone A22106J). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
648832 25 µg 136€
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648833 100 µg 360€
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Description

Visinin-like protein 1 (VILIP-1) is a member of the neuronal calcium sensor (NCS) protein family and plays an essential role in calcium-mediated signal transduction within the human central nervous system. VILIP-1 contains EF-hand motifs that bind calcium ions, leading to conformational changes that allow it to interact with various target proteins such as adenylyl cyclases and membrane-associated signaling molecules. Through these interactions, VILIP-1 modulates cyclic nucleotide signaling and synaptic plasticity, processes fundamental to learning, memory, and neuronal development.

In Alzheimer’s disease (AD), neuronal injury and synaptic dysfunction result in the release of VILIP-1 into the cerebrospinal fluid (CSF), where it can be detected at elevated levels. CSF VILIP-1 levels correlate with biomarkers of neuronal degeneration such as total Tau and phosphorylated Tau, and can predict cognitive decline and progression from mild cognitive impairment (MCI) to AD. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human VILIP-1 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

RRID
AB_3717216 (BioLegend Cat. No. 648832)
AB_3717216 (BioLegend Cat. No. 648833)

Antigen Details

Structure
VILIP-1 is a 191 amino acid length protein with a predicted molecular weight of 22.1 kD.
Distribution

Cytoplasm

Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References
  1. Bernstein HG, et al. 1999. J. Neurocytol. 28:655-62.
  2. Tarawneh R, et al. 2012. Neurology. 78:709-19.
  3. Dulewicz M, et al. 2020. Int. J. Mol. Sci. 21:8335.
Gene ID
7447 View all products for this Gene ID
UniProt
View information about VILIP-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07.25.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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