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Purified anti-TRIM29 Antibody

Pricing & Availability
Clone
W21025C (See other available formats)
Regulatory Status
RUO
Other Names
Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein, ATDC
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W21025C_PURE_TRIM29_WB_060925
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-TRIM29 (clone W21025C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W21025C_PURE_TRIM29_WB_060925
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-TRIM29 (clone W21025C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W21025C_PURE_TRIM29_IHC-P_060925
    IHC staining with Purified anti-TRIM29 (clone W21025C) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using 1X Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704), the tissue was incubated without (panel A) and with (panel B) Purified anti-TRIM29 (clone W21025C) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured on Olympus with a 40X objective and merged. Scale bar: 50 µm
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648551 25 µg 136€
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648552 100 µg 364€
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Description

TRIM29 (Tripartite Motif Containing 29), also known as ATDC (Ataxia-Telangiectasia Group D-Complementing), is a member of the TRIM protein family, which is characterized by a tripartite motif consisting of a RING finger domain, one or two B-box motifs, and a coiled-coil domain. Unlike most TRIM proteins, TRIM29 lacks the typical RING finger domain, which often confers E3 ubiquitin ligase activity. TRIM29 is primarily expressed in epithelial tissues such as the skin, gastrointestinal tract, and respiratory system. In normal physiological conditions, TRIM29 plays a crucial role in maintaining genomic stability and is involved in DNA repair pathways interacting with molecules such as p53-binding protein 1 (53BP1) and TIP60. Through these interactions, TRIM29 helps regulate DNA double-strand break (DSB) repair via the non-homologous end joining (NHEJ) and homologous recombination (HR) pathways. TRIM29 also functions in controlling epithelial cell differentiation and immune responses. TRIM29 can suppress type I interferon responses by regulating the STING pathway, which is essential for antiviral defense. In breast cancer and head and neck squamous cell carcinoma (HNSCC), TRIM29 often acts as an oncogene. Overexpression of TRIM29 in these cancers promotes tumor growth, invasion, and metastasis. Conversely, in gastric and colorectal cancers, TRIM29 act as a tumor suppressor. The expression is often reduced or silenced via promoter hypermethylation. The loss of TRIM29 function may lead to increased genomic instability and tumor progression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human TRIM29
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

RRID
AB_3717123 (BioLegend Cat. No. 648551)
AB_3717123 (BioLegend Cat. No. 648552)

Antigen Details

Structure
Contains 588 amino acids with a predicted molecular weight of 66 kD.
Distribution

Cytoplasm

Cell Type
Epithelial cells, Macrophages
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Ubiquitin/Protein Degradation
Antigen References
  1. Masuda Y, et al. 2015. Nat. Commun. 6:7299.
  2. Xing J. et al. 2016. Nat. Immunol.17:1373-1380.
  3. Li Q, et al. 2018. Cell Discov. 4:13.
Gene ID
23650 View all products for this Gene ID
UniProt
View information about TRIM29 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.09.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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