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Purified anti-mouse Siglec-G Antibody

Pricing & Availability
Clone
W20144D (See other available formats)
Regulatory Status
RUO
Other Names
Siglec-G
Isotype
Rat IgG2b, κ
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Product Citations
publications
W20144D_PURE_-Siglec-G_FC_022625
C57BL/6 mouse splenocytes were stained with purified anti-mouse Siglec-G (clone W20144D) (left) or purified rat IgG2b, κ isotype control (right), followed by goat anti-rat IgG APC and anti-mouse CD19 (clone 6D5) FITC.
  • W20144D_PURE_-Siglec-G_FC_022625
    C57BL/6 mouse splenocytes were stained with purified anti-mouse Siglec-G (clone W20144D) (left) or purified rat IgG2b, κ isotype control (right), followed by goat anti-rat IgG APC and anti-mouse CD19 (clone 6D5) FITC.
  • W20144D_PURE_-Siglec-G_IHC-P_022625
    IHC staining of purified anti-mouse Siglec-G (clone W20144D) on frozen mouse spleen tissue. Frozen mouse spleen tissue sections were treated with Fixation Buffer (Cat. No. 420801), blocked with 5% FBS for 1 hour at room temperature, and incubated with purified anti-mouse Siglec-G (clone W20144D) (panel A) or purified rat IgG2b, κ isotype control (panel B) at 4°C overnight. The tissue was then stained with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 10X objective. Scale bar: 100 µm
Cat # Size Price Quantity Check Availability Save
167902 100 µg 294€
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Description

Siglec-G is a member of the CD33-related Siglec (sialic acid–binding, immunoglobulin-like lectin) family. Siglec-G is the orthologue of human Siglec-10. It is a 110-120 kD, 688 amino acid type I transmembrane protein and contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs. It is an inhibitory receptor that is expressed in a B cell–restricted way, with particularly high expression in Pre-B and B1a cells. Upon stimulation with an antigen, Siglec-G is recruited to IgM, which is further phosphorylated inhibiting IgM-induced Ca2+ signaling and NFATc1 expression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified

IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clones W20144D and SH1 can block the binding of each other.

RRID
AB_3716906 (BioLegend Cat. No. 167902)

Antigen Details

Structure
Type I transmembrane protein that contains five extracellular immunoglobulin domains and an intracellular region with three conserved tyrosine-based motifs.
Distribution

B cells

Function
Inhibitis IgM-induced Ca2+ signaling and NFATc1 expression
Ligand/Receptor
Lectin
Gene ID
243958 View all products for this Gene ID
UniProt
View information about Siglec-G on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02.26.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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