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Purified anti-human MMP-7 Antibody

Pricing & Availability
Clone
Poly23A29 (See other available formats)
Regulatory Status
RUO
Other Names
Matrix metalloproteinase 7 (MMP-7), MMP, Matrilysin, Pump-1 protease, Uterine metallo
Isotype
Goat Polyclonal IgG
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Poly23A29_PURE_MMP-7_ELISA_092425
Direct ELISA showing purified anti-human MMP-7 (clone Poly23A29) bound plate immobilized recombinant human MMP-7 (Cat Nos. 761302, 761304 and 761306). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Doney anti-goat. TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450nm.
  • Poly23A29_PURE_MMP-7_ELISA_092425
    Direct ELISA showing purified anti-human MMP-7 (clone Poly23A29) bound plate immobilized recombinant human MMP-7 (Cat Nos. 761302, 761304 and 761306). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Doney anti-goat. TMB High Sensitivity Substrate Solution (Cat No. 421501) was used for detection. Absorbance was measured at 450nm.
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612161 100 µg 280€
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Description

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological and pathological processes such as morphogenesis, differentiation, angiogenesis, tissue remodeling, and tumor invasion. A typical MMP consists of a pro-peptide of about 80 amino acids, a catalytic metalloproteinase domain of about 170 amino acids, a linker peptide of variable lengths and a hemopexin domain of about 200 amino acids. The zinc binding motif HEXXHXXGXXH in the catalytic domain, and the ‘‘cysteine switch’’ motif PRCGXPD in the pro-peptide are common structural signatures. MMP-7, Matrilysin, is one of the smallest members of the MMP family consisting of a pro-peptide domain and a catalytic domain. It can degrade casein, laminin, fibronectin, collagen type III/IV/V/IX/X/XI, gelatin type I/II/IV/ V, elastin, and proteoglycans. MMP-7 is secreted specifically by epithelial cells and its over-expression has been observed in many tumor types such as colorectal cancer, epidermolysisbullosa associated skin cancer, bladder cancer, gastric cancers, pancreatic cancer and esophageal cancer. This enzyme serves essential functions in both innate defense and wound healing, and appears to be one of the most important MMPs in human colon cancers. It has been reported that MMP-7 contributes to tumor malignancy probably by cleaving cell surface proteins such as Fas ligand (reducing its effectiveness in triggering Fas-mediated apoptosis) and E-cadherin (disruption to the E-cadherin/beta-catenin complex results in a switch to a more invasive phenotype in cancer cells). In addition, degradation of IgG by MMP-7 may protect tumor cells from the immune system, since cleavage of IgG will remove much of the immunoglobulins' functionality.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant Human MMP-7 (carrier-free)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone Poly23A29) could function as a capture and detection antibody as a self-pairing antibody and using Recombinant Human MMP-7 (carrier-free) protein (Cat. Nos. 761302, 761304 and 761306) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

RRID
AB_3720588 (BioLegend Cat. No. 612161)

Antigen Details

Structure
Monomer
Distribution

Neutrophils, macrophages, monocytes

Function
ECM remodeling and wound healing; TIMPs are known inhibitor of MMP-7.
Interaction
Extracellular Matrix (ECM)
Ligand/Receptor
TIMP-1, TIMP-2, TIMP-4
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Enzymes and Regulators
Antigen References
  1. Basu S, et al. 2015. PLoS One. 10:e0123979.
  2. Tallant C, et al. 2010. Biochim. Biophys. Acta. 1803:20.
  3. Nagase H, et al. 2006. Cardiovasc. Res. 69:562.
  4. Rundhaug JE. 2005. J. Cell. Mol. Med. 9:267.
  5. Mott JD, Werb Z. 2004. Curr. Opin. Cell. Biol. 16:558.
  6. Kioi M, et al. 2003. Oncogene. 22(54):8662-70.
Gene ID
4316 View all products for this Gene ID
UniProt
View information about MMP-7 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09.24.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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