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Purified anti-human IDO1 Antibody

Pricing & Availability
Clone
W20099F (See other available formats)
Regulatory Status
RUO
Other Names
Indolamine 2.3-dioxygenase, Indole 2.3-dioxygenase, Indoleamine-pyrrole 2.3 dioxygenase
Isotype
Rat IgG2b, κ
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Product Citations
publications
W20099F_PURE_IDO1_ICC_021126
Human epidermoid carcinoma cell line, A431, was stimulated with 50 ng/mL of recombinant human IFN-γ (Cat. No. 713906) for 48 hours (panels A and C) or left unstimulated (panel B). Cells were fixed, permeabilized, and then stained with 5 µg/mL of purified anti-human IDO1 (clone W20099F) (panels A and B) or purified rat IgG2b, κ isotype control (panel C), followed by anti-rat IgG Alexa Fluor® 594. Nuclei were counterstained with Hoechst 33342. Cells were imaged using a 40X objective. Scale bar = 20 µm
  • W20099F_PURE_IDO1_ICC_021126
    Human epidermoid carcinoma cell line, A431, was stimulated with 50 ng/mL of recombinant human IFN-γ (Cat. No. 713906) for 48 hours (panels A and C) or left unstimulated (panel B). Cells were fixed, permeabilized, and then stained with 5 µg/mL of purified anti-human IDO1 (clone W20099F) (panels A and B) or purified rat IgG2b, κ isotype control (panel C), followed by anti-rat IgG Alexa Fluor® 594. Nuclei were counterstained with Hoechst 33342. Cells were imaged using a 40X objective. Scale bar = 20 µm
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377116 100 µg 220€
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Description

IDO1, also known as indolamine 2,3-dioxygenase, indole 2,3-dioxygenase, and indoleamine-pyrrole 2,3-dioxygenase, is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. It is one of the best-known IFN-γ inducible genes. The IDO1 enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO1 plays an important role in host defense against both intracellular and extracellular pathogens and contributes to the special immunosuppressive status of immune-privileged sites such as the brain, eyes, kidneys, and placenta.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein, full length human IDO, expressed in E.coli
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration of 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
403 amino acids with a predicted molecular weight of 45 kD.
Distribution

Cytoplasm

Function
Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.
Interaction
This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Innate Immunity
Antigen References
  1. Habara-Ohkubo A, et al. 1991. Gene. 105:221.
  2. Munn DH, et al. 2002. Science. 297:1867.
  3. Frumento G, et al. 2002. J. Exp. Med. 196:459.
  4. Muller AJ, et al. 2005. Nature Med. 11:312.
  5. Löb S, et al. 2009. Nature Rev. Cancer. 9:445-52.
Gene ID
15930 View all products for this Gene ID
UniProt
View information about IDO1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09.26.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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