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Purified anti-Fibronectin Antibody

Pricing & Availability
Clone
C6F10 (See other available formats)
Regulatory Status
RUO
Other Names
FN1, FN
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. C6F10_PURE_Fibronectin_WB_091625
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines or recombinant protein (Recombinant Human FN-1, Cat. No. 775306) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Fibronectin (clone C6F10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. C6F10_PURE_Fibronectin_WB_091625
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines or recombinant protein (Recombinant Human FN-1, Cat. No. 775306) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-Fibronectin (clone C6F10) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. C6F10_PURE_Fibronectin_ICC_091625
    HeLa cells (negative cell line, panel A) and HepG2 cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-Fibronectin (clone C6F10), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. C6F10_PURE_Fibronectin_ICFC_091625
    HepG2 cells (positive cell line, filled histogram) and HeLa cells (negative cell line, open histogram) were fixed and permeabilized using True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) and intracellularly stained with Purified anti-Fibronectin (clone C6F10) or Purified Mouse IgG1, κ isotype control (dashed histogram) (Cat No. 400102) followed by Alexa Fluor® 647 anti-mouse IgG1 Antibody (Cat. No. 406618).
Cat # Size Price Quantity Check Availability Save
648870 25 µg 152€
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648871 100 µg 368€
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Description

Fibronectin is a glycoprotein that plays a critical role in cell adhesion, growth, migration, and differentiation. It is a major component of the extracellular matrix (ECM), where it helps to organize other matrix proteins and anchor cells via integrin receptors. Fibronectin is essential during embryonic development and wound healing, facilitating tissue morphogenesis and repair by guiding fibroblast and endothelial cell movement. It also contributes to mechanotransduction and regulates cellular signaling pathways involved in survival and proliferation. Dysregulation of fibronectin expression or structure has been implicated in fibrosis, cancer metastasis, and chronic inflammation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human plasma fibronectin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100
-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only

For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with any of the following:
-Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803)
-True-Phos™ Perm Buffer (Cat. No. 425401)
-True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)

RRID
AB_3720599 (BioLegend Cat. No. 648870)
AB_3720599 (BioLegend Cat. No. 648871)

Antigen Details

Structure
Fibronectin is a 2477 amino acid protein with a predicted molecular weight of 272.3 kD.
Distribution

Secreted, extracellular space

Function
Adhesion molecule
Interaction
Collagen, Fibrin, heparin domains, Tenascin-C, Laminin, degraded by matrix metalloproteinases
Ligand/Receptor
Integrin receptors, syndecans
Cell Type
Fibroblasts, Mesenchymal cells
Biology Area
Angiogenesis, Cell Adhesion
Molecular Family
Adhesion Molecules
Antigen References
  1. Singh P, et al. 2010. Ann Rev Cell Dev. Biol. 26:397-419.
  2. Patten J, Wang K. 2021. Adv Drug Deliv Rev. 70:353-368. 
  3. Sun Y, et al. 2025. J Cell Sci. 138(6):jcs263834.
Gene ID
2335 View all products for this Gene ID
UniProt
View information about FN-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.16.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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