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PE anti-human MRP-14 (S100A9) Antibody

Pricing & Availability
Clone
A22135F (See other available formats)
Regulatory Status
RUO
Other Names
S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A. A22135F_PE_MRP-14_1_101325
Human peripheral blood granulocytes were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No.426803) and intracellularly stained with anti-human CD15 (clone W6D3) Pacific Blue™ and anti-human MRP-14 (S100A9) (clone A22135F) PE (left) or mouse IgG2b, κ PE isotype control (right).
  • A. A22135F_PE_MRP-14_1_101325
    Human peripheral blood granulocytes were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No.426803) and intracellularly stained with anti-human CD15 (clone W6D3) Pacific Blue™ and anti-human MRP-14 (S100A9) (clone A22135F) PE (left) or mouse IgG2b, κ PE isotype control (right).
  • B. A22135F_PE_MRP-14_2_101325
    Human peripheral blood mononuclear cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. No.426803) and intracellularly stained with anti-human CD14 (clone HCD14) Pacific Blue™ and anti-human MRP-14 (S100A9) (clone A22135F) (left) or mouse IgG2b, κ PE isotype control (right). Data shown is gated on the lymphocyte and monocyte populations.
See PE spectral data
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377128 25 tests 105€
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377129 100 tests 233€
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Description

The S100A8/A9 complex has been shown to play a role in calcium-dependent activation and exhibits antibacterial, antifungal, immunomodulatory, and anti-proliferative effects. It also functions as a chemoattractant for neutrophils and serves as an endogenous activator of Toll-like receptor 4 (TLR4). S100A9 has been identified as a marker of CD14⁺ HLA-DR⁻/low myeloid-derived suppressor cells (MDSCs).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein of human MRP-14 (S100A9)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3720634 (BioLegend Cat. No. 377128)
AB_3720634 (BioLegend Cat. No. 377129)

Antigen Details

Structure
S100A9 belongs to S100 family of proteins containing 2-EF-hand calcium-binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8.
Distribution

Secreted and localized to the cytoplasm and cell membrane in cells such as MDSCs, neutrophils, monocyte, and ileal tissue eosinophils

Function
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Cell Type
Eosinophils, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules
Antigen References
  1. Loser K, et al. 2010. Nat. Med. 16:713.
  2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
  3. Leukert N, et al.2006. J. Mol. Biol. 359:961.
  4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
  5. Zhao F, et al. 2012. Immunology. 136:176.
Gene ID
6280 View all products for this Gene ID
UniProt
View information about MRP-14 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 10.13.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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