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PE anti-human Glypican-4 Antibody

Pricing & Availability
Clone
A21050B (See other available formats)
Regulatory Status
RUO
Other Names
GPC4
Isotype
Mouse IgG2b, κ
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Product Citations
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A21050B_PE_Glypica4_FC_092925
The human HAP1-WT cell line was stained with anti-human Glypican-4 (clone A21050B) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • A21050B_PE_Glypica4_FC_092925
    The human HAP1-WT cell line was stained with anti-human Glypican-4 (clone A21050B) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
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386405 25 tests 162€
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Description

Glypican-4 is a cell surface heparan sulfate proteoglycan that contains a core protein anchored to the cytoplasmic membrane via a GPI anchor. Glypicans function as coreceptors for a variety of growth factors including Wnt, bone morphogenetic proteins (BMPs), fibroblast growth factors, and Hedgehog. In particular, Glypican-4 enhances the Wnt3a dependent β-catenin pathway and the Wnt5a-dependent non-canonical Wnt signaling. Glypican-4 plays a role in the control of cell division and growth regulation and is upregulated in pancreatic cancer and colorectal cancer where it is involved in promoting chemoresistance. Glypican-4 also regulates glutamatergic synapse formation and function in the developing brain.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
AA 23-529
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody blocks Glypican-4 mediated Wnt3a signaling in vitro at concentrations from 0.1 - 5.0 µg/mL.

Antigen Details

Structure
Cell surface heparan sulfate proteoglycan
Distribution

Cell membrane and nucleoplasm

Function
Enhances Wnt signaling by acting as R-spondin co-receptor
Interaction
PICK1, Insulin Receptor, Wnt3a, FGF2
Ligand/Receptor
Wnt3a
Cell Type
Neurons
Biology Area
Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Signal Transduction
Molecular Family
APP/Aβ Degradation
Antigen References
  1. Sakane H, et al. 2012. J Cell Sci. 125:449-60.
  2. Hagihara K, et al. 2000. Developmental Dynamics. 219:353-67.
  3. Watanabe K, et al. 1995. J Cell Biol. 130:1207-18.
  4. Ussar, S. et al. 2012. Diabetes. 61:2289-98. 
Gene ID
2239 View all products for this Gene ID
UniProt
View information about Glypican-4 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09.29.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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