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KIRAVIA Blue 520™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin, SAV, SAV
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Product Citations
publications
KB520_Streptavidin_Antibody_060121.png
Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-KIRAVIA Blue 520™ (filled histogram), or biotinylated mouse IgG1 isotype control, followed with SAV-KIRAVIA Blue 520™ (open histogram).
  • KB520_Streptavidin_Antibody_060121.png
    Human peripheral blood lymphocytes were stained with biotinylated CD3, followed by SAV-KIRAVIA Blue 520™ (filled histogram), or biotinylated mouse IgG1 isotype control, followed with SAV-KIRAVIA Blue 520™ (open histogram).
See KIRAVIA Blue 520™ spectral data
Cat # Size Price Quantity Check Availability Save
405172 100 µg 90€
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Description

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
Concentration
0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.


KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Sony and the Sony logo are registered trademarks of Sony Corporation.
Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-KIRAVIA Blue 520™ is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-KIRAVIA Blue 520™ is 65 kD and Streptavidin alone is 52 kD.

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Go To Top Version: 2    Revision Date: 11.13.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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