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Spark PLUS UV395™ Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin- Spark PLUS UV395™, SAv- Spark PLUS UV395™, streptavidin Spark PLUS UV395™, SAV
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Product Citations
publications
SparkPLUSUV395_SAV_anti-human_032726
Human peripheral blood cells were stained with anti-human CD4 (clone SK3) Alexa Fluor® 700 and biotinylated anti-human CD3 (clone UCHT1), followed by SAV-Spark PLUS UV395™, or stained with anti-human CD4 (clone SK3) Alexa Fluor® 700 only (right). Data shown was gated on lymphocytes.
  • SparkPLUSUV395_SAV_anti-human_032726
    Human peripheral blood cells were stained with anti-human CD4 (clone SK3) Alexa Fluor® 700 and biotinylated anti-human CD3 (clone UCHT1), followed by SAV-Spark PLUS UV395™, or stained with anti-human CD4 (clone SK3) Alexa Fluor® 700 only (right). Data shown was gated on lymphocytes.
See Spark PLUS UV™ 395 spectral data
Cat # Size Price Quantity Check Availability Save
405391 25 µg 156€
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405392 100 µg 344€
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Description

Streptavidin is a 52 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with Spark PLUS UV395™ under optimal conditions
Concentration
0.2 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark PLUS UV395™ conjugation. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.

Excitation Laser
Ultraviolet Laser (355 nm)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.20.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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