Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Denmark (DKK) DKK
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Norway (NOK) NOK
Sweden (SEK) SEK
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- SIRPA, SHP substrate 1, SHPS-1, CD172a, Brain Ig-like moleculewithtyrosine-based activation motifs (Bit), CD172 antigen-likefamilymember A, Inhibitory receptor SHPS-1, Macrophagefusionreceptor, MyD-1 antigen
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant Human CD47 binds to immobilized Recombinant Human SIRPα/CD172a V2 in a dose-dependent manner. ED50 is between 0.4 – 2.4 µg/mL. -
Stability Testing for Recombinant Human SIRPα/CD172a V2. Recombinant Human SIRPα/CD172a V2 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability in a binding assay with Recombinant Human CD47. The ED50 for this effect is 0.4 – 2.4 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 711854 | 25 µg | 148€ | ||||
| 711856 | 100 µg | 312€ | ||||
Signal Regulatory Proteins (SIRPs) are a family of transmembrane glycoproteins with extracellular IgG domains. The extracellular regions of the SIRPs are comprised of three Ig-like loops; the proximal loop contains two IgC domains while the distal membrane loop contains one IgV domain. While these extracellular domains are similar among SIRPs, their transmembrane and cytoplasmic domains differentiate them from one another. Signal regulatory protein α (SIRPα) is highly expressed in macrophages and dendritic cells. Interaction of the extracellular region of SIRPα with its ligand CD47 elicits signaling and SIRPα phosphorylation. CD47-SIRPα signaling results in the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2, which negatively regulate cellular functions, including the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion and phagocytosis. In addition to its role in "self-signaling", SIRPα also functions as a scaffold for membrane-bound signaling molecules. Interaction of CD47 on tumor cells with SIRPα on tumor-associated macrophages (TAMs) constitutes an innate immune checkpoint that interferes with the detection and phagocytosis. SIRPα V1, SIRPα V2, and SIRPα V8 are the main SIRPα variants in humans. hSIRPα V1 is dominant among Europeans, Africans, and South Asians while hSIRPα V2 dominates east Asians. Sequence alignment of hSIRPα V1, hSIRPα V2, and hSIRPα V8 proteins demonstrated the differences within the CD47-binding extracellular Ig-like V-type (IgV) domain.
Product DetailsProduct Details
- Source
- Human SIRPα V2, amino acid (Glu31-Arg369) (Accession # AAH38510.1), with a C-terminal His tag, was expressed in 293E.
- Molecular Mass
- The 345 amino acid recombinant protein has a predicted molecular mass of approximately 37.98 kD. The DTT-reduced and non-reduced protein shall migrate at approximately 40 - 70 kD by SDS-PAGE. The predicted N-terminal amino acid is Glu.
- Purity
- > 95%, as determined by Coomassie-stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When Recombinant Human SIRPα/CD172a V2 is immobilized at 0.2 µg/mL, Recombinant Human CD47 (Cat. No. 785406) binds in a dose-dependent manner with an ED50 range of 0.4 – 2.4 µg/mL. Biotin anti-human CD47 Antibody (Cat. No. 323104) and HRP-Avidin (Cat. No. 405103) was used to detect the binding.
- Application
-
Biossay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Macrophages and dendritic cells
- Function
- Regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion and phagocytosis
- Interaction
- CD47-expressing cells including tumor cells
- Ligand/Receptor
- CD47
- Bioactivity
- Recombinant human CD47 binding to Immobilized recombinant human SIRPα/CD172a V2 in a dose-dependent manner. ED50 of this effect is 0.4 – 2.4 µg/mL.
- Cell Type
- Macrophages, Neurons
- Biology Area
- Cell Adhesion, Innate Immunity, Neuroscience
- Molecular Family
- Adhesion Molecules, Soluble Receptors
- Antigen References
-
- Van Beek EM, et al. 2005. J Immunol. 12:7781.
- Lee WY, et al. 2007. J Immunol. 11:7741.
- Subramanian S, et al. 2006. Blood. 107:2548.
- Barclay AN. 2009. Current Opinion in Immunology. 21(1):47.
- Liu Y, et al. 2005. J Biol Chem. 43:36132.
- Takenaka K, et al. 2007. Nat Immunol. 12:1313.
- Veillette A, et al. 2018. Trends Immunol. 39:173.
- Barclay AN, et al. 2014. Annu Rev Immunol. 32:25.
- Murata Y, et al. 2018. Cancer Sci. 109:2349.
- Matozaki T, et al. 2009. Trends Cell Biol. 19:72.
- Feng M, et al. 2019. Nat Rev Cancer. 19:568.
- Voets E, et al. 2019. J Immunother Cancer. 7:340.
- Gene ID
- 140885 View all products for this Gene ID
- UniProt
- View information about SIRPalpha/CD172a on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
-
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
-
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
-
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
-
Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
Follow Us