Loading...

Purified anti-S100A10 Antibody

Pricing & Availability
Clone
6F4-E6-D5-C10 (See other available formats)
Regulatory Status
RUO
Other Names
Protein S100-A10 (S100A10), Calpactin I light chain (CAL1L), cellular ligand of Annexin II (ANX2LG), S100 calcium-binding protein A10
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
A. 6F4-E6-D5-C10_PURE_-S100A10_IHC-P_082125
IHC staining of Purified anti-S100A10 (clone 6F4-E6-D5-C10) on formalin-fixed paraffin-embedded human cortex tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) Purified anti-S100A10 (clone 6F4-E6-D5-C10) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a 40X objective and merged. Scale bar: 50 µm
  • A. 6F4-E6-D5-C10_PURE_-S100A10_IHC-P_082125
    IHC staining of Purified anti-S100A10 (clone 6F4-E6-D5-C10) on formalin-fixed paraffin-embedded human cortex tissue. Following antigen retrieval using 1X Citrate Buffer (Cat. No. 420902), the tissue was incubated without (panel A) and with (panel B) Purified anti-S100A10 (clone 6F4-E6-D5-C10) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured using a 40X objective and merged. Scale bar: 50 µm
  • B. 6F4-E6-D5-C10_PURE_-S100A10_ICC_082125
    SH-SY5Y cells (negative cell line, panel A) and HaCat cells (positive cell line, panel B) were fixed with 4% PFA Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with Purified anti-S100A10 (clone 6F4-E6-D5-C10), followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective and merged. Scale bar: 50 µm
  • C. 6F4-E6-D5-C10_PURE_-S100A10_WB_082125
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-S100A10 (clone 6F4-E6-D5-C10) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • D. 6F4-E6-D5-C10_PURE_-S100A10_ICFC_082125
    HaCat cells (positive cell line, filled histogram) and SH-SY5Y cells (negative cell line, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Purified anti-S100A10 (clone 6F4-E6-D5-C10), or Purified Mouse IgG2a, κ Isotype Control (open histogram, dashed line, representative of both the positive and negative cell lines) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322).
Cat # Size Price Quantity Check Availability Save
648840 25 µg 128€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
648841 100 µg 352€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

S100A10 is a member of the S100 family of proteins found in vertebrates with over 20 known members. S100A10 primarily functions as a regulator of membrane-related processes, including endocytosis and exocytosis, by forming a complex with annexin A2. This complex facilitates the trafficking and surface expression of ion channels, such as the epithelial sodium channel and certain TRP channels. S100A10 is also involved in plasminogen activation and fibrinolysis by promoting the generation of plasmin on the cell surface. Additionally, it has roles in modulating serotonin signaling and has been implicated in mood regulation and depression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human S100A10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.0625 - 2.5 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 2.5 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.015 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional Product Notes

For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:
-Fixation Buffer (Cat. No. 420801) with 100% ice-cold methanol
-100% ice-cold methanol only
Fixation/permeabilization with Fixation Buffer followed by 0.5 % Triton X-100 is not recommended due to dim signal. 

For use in intracellular flow cytometry (ICFC), we recommend fixation/permeabilization using fixation buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat. No. 425401). Fixation/permeabilization with either Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-NuclearTM Transcription Factor Buffer Set (Cat. No. 424401) is not recommended.

RRID
AB_3720560 (BioLegend Cat. No. 648840)
AB_3720560 (BioLegend Cat. No. 648841)

Antigen Details

Structure
S100A10 is a 97 amino acid protein with a predicted molecular weight of 11.2 kD.
Distribution

Cell surface, cytoplasm

Function
Scaffold protein
Interaction
Forms heterotetramers consisting of two molecules of S100A10 and two of Annexin A2 (ANXA2). Interacts with many transmembrane and membraneassociated proteins such as AHNAK, TRP channels, serotonin receptors (5- HT), et al.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Stem Cells
Molecular Family
Neurotransmitter Receptor Trafficking/Endocytosis, Scaffold Proteins
Antigen References
  1. Okura GC, et al. 2013. Biomolecules. 13(10):1450.
  2. Chen MX, et al. 2022. Front Mol Neurosci. 15:953066.
  3. Johnsson N, et al. 1986. FEBS Lett. 198(2):361-4.
Gene ID
6281 View all products for this Gene ID
UniProt
View information about S100A10 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All S100A10 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-S100A10 6F4-E6-D5-C10 IHC-P,ICC,WB,ICFC
Go To Top Version: 1    Revision Date: 08.21.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account