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PE anti-mouse CD146 Antibody

Pricing & Availability
Clone
29-1709 (See other available formats)
Regulatory Status
RUO
Other Names
CD146, S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
29-1709_PE_CD146_FC_062725
C57BL/6 splenocytes were stained with anti-mouse NK-1.1 (clone PK136) Alexa Fluor® 647 and anti-mouse CD146 (clone 29-1709) PE (left) or rat IgG2b, κ PE isotype control (right).
  • 29-1709_PE_CD146_FC_062725
    C57BL/6 splenocytes were stained with anti-mouse NK-1.1 (clone PK136) Alexa Fluor® 647 and anti-mouse CD146 (clone 29-1709) PE (left) or rat IgG2b, κ PE isotype control (right).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
168403 25 µg 117€
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168404 100 µg 223€
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Description

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is a cell adhesion molecule that plays a role in cell-cell and cell-extracellular matrix interactions and its expression on vascular endothelial basement membranes facilitates TH17 cell entry into tissues. CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1-positive cells, and neutrophils. In mice, CD146 is a marker of natural killer cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3717151 (BioLegend Cat. No. 168403)
AB_3717151 (BioLegend Cat. No. 168404)

Antigen Details

Structure
CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
Distribution

CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.

Function
CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
Interaction
Endothelial cells, Mesenchymal Stem Cells, Neutrophils
Ligand/Receptor
Laminin-411
Cell Type
Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Despoix N, et al. 2008. Eur. J. Immunol. 38:2855.
  2. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
  3. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.
  4. Flanagan K, et al. 2012. PLOS One. 7:e40443.
Gene ID
84004 View all products for this Gene ID
UniProt
View information about CD146 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06.27.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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