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APC anti-human CD279 (PD-1) Recombinant Antibody

Pricing & Availability
Clone
MIH4.Rec (See other available formats)
Regulatory Status
RUO
Other Names
PD1, P:D-1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MIH4Rec_APC_CD279_Antibody_1_032125-3
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) Pacific Blue™ and anti-human CD279 (PD-1) recombinant (clone MIH4.Rec) APC (left) or mouse IgG1, κ APC isotype control (right).
  • MIH4Rec_APC_CD279_Antibody_1_032125-3
    Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) Pacific Blue™ and anti-human CD279 (PD-1) recombinant (clone MIH4.Rec) APC (left) or mouse IgG1, κ APC isotype control (right).
  • MIH4Rec_APC_CD279_Antibody_2_042125-2
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD279 (PD-1) recombinant (clone MIH4.Rec) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram)
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Cat # Size Price Quantity Check Availability Save
335705 25 tests 128€
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335706 100 tests 285€
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Description

CD279, also known as Programmed cell Death 1 (PD-1), is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 and are members of the B7 gene family. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

MIH4.rec clone does not affect the binding of clones NAT105, EH12.2H7, A17188A, and A17188B on target cells.

RRID
AB_3698987 (BioLegend Cat. No. 335705)
AB_3698987 (BioLegend Cat. No. 335706)

Antigen Details

Structure
55 kD, immunoglobulin superfamily
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells

Function
Signaling, co-stimulation (co-inhibition)
Interaction
SHP-1 and SHP-2.
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273)
Cell Type
Lymphocytes, T cells
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Francisco LM, et al. 2010. Immunological Rev. 236:219.
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.21.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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