Loading...

RBC Lysis Buffer (10X)

Pricing & Availability
Regulatory Status
RUO
Other Names
Red blood cell lysis buffer
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420301 100 mL 52€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420302 500 mL 165€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  2. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  3. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  4. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  5. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  6. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  7. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  8. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  9. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  10. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  11. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  12. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  13. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  14. Rathakrishnan A, et al. 2020. Viruses. 12: . PubMed
  15. Wang Y, et al. 2021. Cancer Res. 81:384. PubMed
  16. Wang H, et al. 2022. Nat Biomed Eng. 6:882. PubMed
  17. Yeh PY, et al. 2022. Sens Actuators B Chem. 353: . PubMed
  18. Ammothumkandy A, et al. 2022. Nat Neurosci. 25:493. PubMed
  19. Melendez E, et al. 2022. Development. 149: . PubMed
  20. Argueta LB, et al. 2022. iScience. 25:104223. PubMed
  21. Wang S, et al. 2022. Adv Sci (Weinh). 9:e2201258. PubMed
  22. Meena AS, et al. 2022. Cell Rep. 39:110937. PubMed
  23. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  24. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  25. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  26. Isildar B, et al. 2022. Inflamm Regen. 42:55. PubMed
  27. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  28. Alexandrou AT, et al. 2022. iScience. 25:105546. PubMed
  29. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  30. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  31. Tratwal J, et al. 2022. Front Endocrinol (Lausanne). 13:1001210. PubMed
  32. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
  33. Xiang X, et al. 2022. Medicine (Baltimore). 101:e32558. PubMed
  34. Yu Z, et al. 2023. Cancer Res. 83:700. PubMed
  35. Ding D, et al. 2023. J Med Chem. 66:1467. PubMed
  36. Oh S, et al. 2023. Nat Biotechnol. :. PubMed
  37. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  38. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  39. Vyas M, et al. 2023. Front Immunol. 13:1098445. PubMed
  40. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  41. Wang J, et al. 2023. Inflamm Regen. 43:12. PubMed
  42. Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed
  43. Feng J, et al. 2022. Immunity. 55:405. PubMed
  44. Rustagi Y, et al. 2022. Diabetes. 71:1149. PubMed
  45. Guennoun R, et al. 2022. Cancers (Basel). 14:. PubMed
  46. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  47. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  48. Wakiyama H, et al. 2022. Cancer Immunol Res. 10:1386. PubMed
  49. Roweth HG, et al. 2022. Sci Adv. 8:eabo5224. PubMed
  50. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  51. Yun HJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  52. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  53. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
  54. Le DT, et al. 2023. iScience. 26:106059. PubMed
  55. Romo-Rodríguez R, et al. 2023. Arch Med Res. 54:197. PubMed
  56. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  57. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
  58. Chen Z, et al. 2023. Nat Commun. 14:1839. PubMed
  59. Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed
  60. Ramalingam P, et al. 2023. Nat Commun. 14:2018. PubMed
  61. Choi YJ, et al. 2023. Nat Commun. 14:2045. PubMed
  62. He Q, et al. 2023. Parasit Vectors. 16:139. PubMed
  63. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  64. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  65. Villalba N, et al. 2023. J Neuroinflammation. 20:127. PubMed
  66. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  67. Matsudaira T, et al. 2023. Commun Biol. 6:665. PubMed
  68. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  69. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  70. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  71. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  72. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  73. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  74. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  75. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  76. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  77. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  78. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  79. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  80. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  81. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  82. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  83. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  84. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  85. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  86. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  87. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  88. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  89. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  90. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  91. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  92. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  93. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  94. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  95. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  96. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  97. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  98. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  99. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  100. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  101. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  102. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  103. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  104. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  105. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  106. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  107. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  108. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  109. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  110. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  111. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  112. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  113. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  114. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  115. Meng S, et al. 2022. Exp Anim. . PubMed
  116. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  117. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  118. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  119. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  120. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  121. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  122. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  123. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  124. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  125. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  126. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  127. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  128. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  129. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  130. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  131. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  132. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  133. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  134. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  135. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  136. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  137. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  138. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  139. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  140. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  141. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  142. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  143. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  144. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  145. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  146. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  147. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  148. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  149. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  150. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  151. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  152. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  153. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  154. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  155. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  156. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  157. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  158. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  159. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  160. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  161. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  162. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  163. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  164. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  165. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  166. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  167. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  168. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  169. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  170. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  171. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  172. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  173. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  174. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  175. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  176. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  177. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  178. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  179. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  180. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  181. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  182. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  183. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  184. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  185. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  186. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  187. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  188. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  189. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  190. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  191. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  192. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  193. Sci T 2013. Toxicol Sci. 136:72. PubMed
  194. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  195. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  196. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  197. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  198. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  199. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  200. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  201. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  202. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  203. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  204. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  205. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  206. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  207. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  208. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  209. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  210. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  211. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  212. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  213. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  214. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  215. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  216. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  217. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  218. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  219. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  220. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  221. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  222. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  223. Zhao J, et al. 2013. Elife. 21:537. PubMed
  224. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  225. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  226. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  227. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  228. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  229. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  230. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  231. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  232. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  233. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  234. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  235. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  236. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  237. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  238. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  239. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  240. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  241. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  242. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  243. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  244. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  245. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  246. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  247. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  248. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  249. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  250. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  251. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  252. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  253. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  254. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  255. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  256. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  257. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  258. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  259. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  260. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  261. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  262. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  263. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  264. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  265. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  266. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  267. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  268. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  269. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  270. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  271. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  272. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  273. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  274. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  275. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  276. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  277. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  278. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  279. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  280. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  281. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  282. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  283. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  284. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  285. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  286. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  287. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  288. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  289. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  290. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  291. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  292. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  293. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  294. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  295. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  296. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  297. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  298. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  299. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  300. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  301. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  302. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  303. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  304. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  305. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  306. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  307. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  308. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  309. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  310. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  311. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  312. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  313. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  314. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  315. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  316. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  317. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  318. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  319. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  320. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  321. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  322. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  323. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  324. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  325. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  326. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  327. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  328. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  329. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  330. Trial J, et al. 2020. Geroscience. . PubMed
  331. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  332. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  333. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  334. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  335. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  336. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  337. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  338. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  339. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  340. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  341. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  342. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  343. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  344. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  345. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  346. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  347. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  348. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  349. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  350. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  351. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  352. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  353. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  354. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  355. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  356. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  357. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  358. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  359. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  360. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  361. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  362. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  363. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  364. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  365. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  366. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  367. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  368. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  369. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  370. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed
  371. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  372. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  373. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  374. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  375. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  376. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  377. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  378. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  379. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  380. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  381. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  382. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  383. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  384. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  385. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  386. Wan C, et al. 2021. Cancer Res. 81:158. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account