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Human Expandable Base Panel (15c)

 

A 15-color expandable panel for human immune cell phenotyping, enabling detailed characterization of monocytes, macrophages, NK and NKT cells, T cells, B cells, dendritic cells, basophils, and ILCs. The panel is designed to keep channels with broad conjugate availability open, allowing seamless customization. Several strategically placed drop-in/open spots enable the addition of extra markers or conjugates, supporting straightforward expansion up to 23 colors without disrupting the core panel design.

 

• Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
• Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
• Product Name: Human Expandable Base Panel (15c)

 

Marker, Fluorophore, and Clone List:

 

Product	Clone
Spark PLUS UV395™ anti-human CD8	RPA-T8	View Product
Brilliant Violet 510™ anti-human CD3	SK7	View Product
Brilliant Violet 570™ anti-human CD20	2H7	View Product
Brilliant Violet 750™ anti-human CD56 (NCAM)	5.1H11	View Product
Spark Blue™ 550 anti-human CD14	63D3	View Product
Spark Blue™ 574 anti-human CD45	2D1	View Product
PerCP/Cyanine5.5 anti-human CD16	B73.1	View Product
PerCP/Fire™ 806 anti-human CD4	SK3	View Product
Spark YG™ 593 anti-human CD19	HIB19	View Product
PE/Fire™ 700 anti-human CD25	M-A251	View Product
PE/Fire™ 810 anti-human HLA-DR	L243	View Product
Spark NIR™ 685 anti-human CD123	S18016F	View Product
APC/Fire™ 750 anti-human CD11c	Bu15	View Product
APC/Fire™ 810 anti-human CD127 (IL-7Rα)	A019D5	View Product
Zombie NIR™ Fixable Viability Kit		View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)		View Product
Cell Staining Buffer		View Product
True-Stain Monocyte Blocker™		View Product
Compensation Beads		View Product

 

15-Color Base Panel

Representative data is shown below. Human PBMCs were stained with the 15-color expandable panel, acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora, and analyzed using CytoScribe™ software.

 

 

23-Color Expanded Panel

Representative data is shown below. Human PBMCs were stained with the an expaneded, 23-color panel, acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora, and analyzed using CytoScribe™ software.